Transcriptional control in Drosophila.
Transcriptional control in Drosophila.
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DOI：
10.1159/000070462
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发表时间：
2003-01-01
期刊：
影响因子：
--
通讯作者：
Sharp, David H.
中科院分区：
文献类型：
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作者：
Reinitz, John;Hou, Shuling;Sharp, David H.
我们提出了一种新的转录控制模型。该模型的中心目标是展示模块化增强子如何从结合位点组中产生。该模型具有三层组织。第一层描述了激活剂和阻遏物与基因调控区域的结合，并结合了竞争和猝灭的抑制作用。第二层描述了与 DNA 结合激活剂结合的接头分子，并结合了直接抑制的效果。最后，转录的激活通过阿累尼乌斯机制进行建模，其中激活接头降低了激活能垒。我们证明该模型可以根据早期果蝇胚胎的转录数据进行测试。我们相信这个模型已经足够完善，可以真实地解释调节分子复杂相互作用的生理后果。本方法以一种重要的方式补充和支持了对多基因调控的见解，这些见解源自旨在制定调控网络逻辑设计原则的工作。
We present a new model of transcriptional control. A central goal of this model is to show how modular enhancers arise from groups of binding sites. The model has a three-layer organization. The first layer describes the binding of activators and repressors to the regulatory region of a gene and incorporates the effects of repression by competition and quenching. The second layer describes adapter molecules binding to DNA-bound activators, and incorporates the effect of direct repression. Finally, the activation of transcription is modeled by an Arrhenius mechanism in which activating adapters lower the activation energy barrier. We show that this model is testable against transcription data derived from early Drosophila embryos. We believe this model is sufficiently refined to give a realistic account of the physiological consequences of complex interactions of regulatory molecules. The present approach supplements and supports, in an essential way, the insights, into multigenic regulation derived from work aimed at formulating logical design principles for regulatory networks.