Phylogenetic position of Diplostomum spp. from New World herons based on complete mitogenomes, rDNA operons, and DNA barcodes, including a new species with partially elucidated life cycle
Phylogenetic position of Diplostomum spp. from New World herons based on complete mitogenomes, rDNA operons, and DNA barcodes, including a new species with partially elucidated life cycle
复制标题
DOI：
10.1007/s00436-020-06713-4
复制
发表时间：
2020-05-30
影响因子：
2
通讯作者：
Takemoto, Ricardo M.
中科院分区：
文献类型：
--
作者：
Locke, Sean A.;Drago, Fabiana B.;Takemoto, Ricardo M.
鹭口病Diplostomum ardeae Dubois，1969自描述以来，在我国的大蓝鹭（Ardea herodias L. 1758年，在美国。本研究中从黑冠夜鹭（Nycticorax nycticorax（L.，1758））在波多黎各的数据匹配D. ardeae从A.在典型区域的Herodias。我们还获得了DNA条形码从形态相似的双体虫从一个rufescent虎鹭（Tigrisoma lineatum（Boddaert，1783））和囊蚴从眼睛晶状体的Trachelyopterus galeatus（Linnaeus，1766）从巴拉那河流域，分别在阿根廷和巴西。在这些南美成虫和幼虫标本中以及最近发表的来自同一地区的11种其他鲶形鱼类的囊蚴序列中，条形码匹配（97-100%同一性）。来自南美物种的条形码，我们将其描述为Diplostomum lunaschiae n.例如，与D. ardeae的差异为7.2- 9.8%，新种与D.鹭科鹭科与先前的CO 1序列系统发育分析一样，D. ardeae和D.新月形; sp.与复口吸虫属（Diplostomum）无关联。在对核rDNA和线粒体基因组进行更多特征丰富的分析中，D. Ardeae是Diplostomum种的分支的早期分歧成员。因此，我们继续考虑D。ardeae和D.露纳斯基亚埃sp.成员的Diplostomum，在最近的建议，这些物种可能属于不同的属。
Diplostomum ardeae Dubois, 1969 has seldom been reported since its description from the great blue heron (Ardea herodias L., 1758) in the USA. Sequences obtained in this study from the barcode region of cytochrome c oxidase 1 (CO1) in diplostomids from black-crowned night heron (Nycticorax nycticorax (L., 1758)) in Puerto Rico matched data from D. ardeae from A. herodias in the type region. We also obtained DNA barcodes from morphologically similar diplostomids from a rufescent tiger heron (Tigrisoma lineatum (Boddaert, 1783)) and from metacercariae from eye lenses of Trachelyopterus galeatus (Linnaeus, 1766) from the Parana River basin in Argentina and Brazil, respectively. Barcodes matched (97-100% identity) in these South American adult and larval specimens as well as in recently published sequences from metacercariae from 11 other siluriform fishes from the same region. Barcodes from the South American species, which we describe as Diplostomum lunaschiae n. sp., differed from those of D. ardeae by 7.2-9.8%, and the new species differs from D. ardeae in its size, pharynx:oral sucker length ratio, egg:body length ratio, and distribution of vitellaria. As in prior phylogenetic analysis of CO1 sequences, both D. ardeae and D. lunaschiae n. sp. were not associated with Diplostomum. In more character-rich analyses of nuclear rDNA and of mitochondrial genomes, D. ardeae was an early divergent member of clades of species of Diplostomum. Consequently, we continue to consider D. ardeae and D. lunaschiae n. sp. members of Diplostomum, in contrast to recent suggestions that these species may belong to a different genus.