Monocyte adherence to fibronectin: role of CD11/CD18 integrins and relationship to other monocyte functions.
Monocyte adherence to fibronectin: role of CD11/CD18 integrins and relationship to other monocyte functions.
复制标题
单核细胞对纤连蛋白的粘附：CD11/CD18 整合素的作用以及与其他单核细胞功能的关系。
DOI：
10.1002/jlb.51.4.400
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发表时间：
1992
影响因子：
5.5
通讯作者：
Stockley,RA
中科院分区：
文献类型：
--
作者：
Owen,CA;Campbell,EJ;Stockley,RA
单核细胞粘附于细胞外基质成分对其在感染部位的积累至关重要。为了深入了解调节单核细胞募集的因素，我们研究了细菌脂多糖（LPS）对单核细胞粘附的调节作用及其机制；此外，我们还对比了贴壁细胞和非贴壁细胞的表型。我们的研究结果表明，只有一小部分单核细胞（20-25%）自发粘附在纤维连接蛋白上，LPS刺激粘附细胞的比例增加了三倍。基础粘附和lps刺激的单核细胞对纤维连接蛋白的粘附主要由CD11/CD18整合素介导。进一步的研究表明，自发贴壁的单核细胞的吞噬活性比非贴壁细胞高14倍，释放的超氧阴离子多1.6倍，含有的过氧化物酶活性比非贴壁细胞高20倍，而lps贴壁细胞具有中间表型。这些结果表明，LPS可能会增加具有抗菌表型的单核细胞的积累，从而促进组织感染的解决。
Adherence of monocytes to extracellular matrix components is critical for their accumulation at sites of infection. To gain insight into the factors that regulate monocyte recruitment, we have studied monocyte adherence with regard to the regulatory effects of bacterial lipopolysaccharide (LPS) and the mechanisms involved; moreover, we have contrasted the phenotypes of adherent and nonadherent cells. Our results show that only a minor subpopulation of monocytes (20–25%) adhere spontaneously to fibronectin and that LPS stimulated a threefold increase in the proportion of adherent cells. Basal adherence and LPS-stimulated adherence of monocytes to fibronectin were substantially mediated by CD11/CD18 integrins. Further studies revealed that spontaneously adherent monocytes were 14-fold more actively phagocytic, released 1.6-fold more superoxide anion, and contained 20-fold more peroxidase activity than nonadherent cells, whereas LPS-adherent cells had an intermediate phenotype. These results indicate that LPS may enhance the accumulation of monocytes with an antimicrobial phenotype and thereby promote resolution of tissue infection.