EXTRACTION AND IDENTIFICATION OF ESSENTIAL OIL FROM CINNAMOMUM ZEYLANICUM BARKS AND STUDY THE ANTIBACTERIAL ACTIVITY
EXTRACTION AND IDENTIFICATION OF ESSENTIAL OIL FROM CINNAMOMUM ZEYLANICUM BARKS AND STUDY THE ANTIBACTERIAL ACTIVITY
复制标题
DOI：
10.15414/jmbfs.2017/18.7.7.312-316
复制
发表时间：
2017-12-01
影响因子：
0.9
通讯作者：
Al-Sahlany, Shayma Thyab Gddoa
中科院分区：
文献类型：
--
作者：
Al-fekaiki, Dhia F.;Niamah, Alaa Kareem;Al-Sahlany, Shayma Thyab Gddoa
本研究旨在通过GC-MS分析鉴定锡兰肉桂树皮中的精油化合物，并通过琼脂扩散法评估精油对单核细胞增生李斯特氏菌、大肠杆菌、产气肠杆菌、产气假单胞菌和金黄色葡萄球菌的抑制效果，并研究对这些细菌的杀灭时间。锡兰肉桂精油的收率为5%。精油提取物中的主要化合物为肉桂醛57.83%，其次是环己烷甲酸9.29%和6-十八碳烯酸8.41%。添加6、12和18μL精油后，革兰氏阳性菌和革兰氏阴性菌的生长均受到抑制。细菌测试的抑菌圈直径范围为17.00-30.30 mm。浓度为10、20和30μL.mL(-1)的精油引起活细胞数量从2Log到5Log的快速稳定下降。所有菌株在 24 小时内循环。
This study aimed to identify the essential oil compound from Cinnamomum zeylanicum barks by using GC-MS analysis and evaluate essential oil inhibition effects against Listeria monocytogenes, Escherichia colt, Enterobacter aerogenes, Pseudomonas erogenous and Staphylococcus aureus by using agar diffusion method and studied kill-time for this bacterium. The yield of Cinnamomum zeylanicum essential oil was 5%. Cinnamaldehyde 57.83% was the main compound in the essential oil extract, followed by cyclohexane carboxylic acid 9.29% and 6-octadecenoic acid 8.41%. Gram-positive and Gram-negative bacteria were inhibited growth after addition 6, 12 and 18 mu L of essential oil. Zone diameter of inhibition ranged from 17.00-30.30 mm for bacterial test. A concentration of 10, 20 and 30 mu L.mL(-1) of the essential oil caused a rapid and steady decline in the number of viable cells from 2 to 5 Log. cycles of all strains during 24 hours.