High resolution neutron protein crystallography. Hydrogen and hydration in proteins
High resolution neutron protein crystallography. Hydrogen and hydration in proteins
复制标题
高分辨率中子蛋白质晶体学。
DOI：
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
I. Tanaka
中科院分区：
文献类型：
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作者：
N. Niimura;T. Chatake;A. Ostermann;K. Kurihara;I. Tanaka
摘要 中子衍射提供了直接定位蛋白质中氢原子的实验方法，中子成像板（NIP）的发展成为中子蛋白质晶体学的突破性事件。回顾了 NIP 的一般特征。日本原子能研究所建造了一台专用于生物大分子的高分辨率中子衍射仪 (BIX-3)，具有 NIP，这使得能够对多种蛋白质进行 1.5 Å 分辨率的结构分析。对 BIX-3 和 LADI（安装在劳厄-朗之万研究所的准劳厄型衍射仪）的规格进行了比较。肌红蛋白、野生型红氧还蛋白和红氧还蛋白突变体的晶体结构已使用 BIX-3 进行了分析。从这些研究中，我们对氢键和某些酸性氢原子的位置、甲基氢原子的识别、H/D交换的细节和水合结构的动力学行为等几个主题进行了研究，并从结构结果中提取了重要信息。最后，描述了生长大的蛋白质或核酸单晶的系统程序。
Abstract Neutron diffraction provides an experimental method of directly locating hydrogen atoms in proteins, and the development of the neutron imaging plate (NIP) became a breakthrough event in neutron protein crystallography. The general features of the NIP are reviewed. A high resolution neutron diffractometer dedicated to biological macromolecules (BIX-3) with the NIP has been constructed at Japan Atomic Energy Research Institute and this has enabled 1.5 Å resolution structural analyses of several proteins to be carried out. The specifications of BIX-3 and LADI (a quasi-Laue type diffractometer installed in the Institut Laue-Langevin) are compared. The crystal structures of myoglobin, wild type rubredoxin and a mutant of rubredoxin have been carried out using BIX-3. From these studies, several topics, such as the location of hydrogen bonds and certain acidic hydrogen atoms, the identification of methyl hydrogen atoms, details of H/D exchange and dynamical behavior of hydration structures have been investigated, and important information has been extracted from the structural results. Finally, a systematic procedure to grow large single crystals of proteins or nucleic acids is described.