Trivalent chromium removal from tannery effluent using kaolin-supported bacterial biofilm of Bacillus sp isolated from chromium polluted soil
Trivalent chromium removal from tannery effluent using kaolin-supported bacterial biofilm of Bacillus sp isolated from chromium polluted soil
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DOI：
10.1002/jctb.2710
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发表时间：
2012-02-01
影响因子：
3.4
通讯作者：
Unni Nair, Balachandran
中科院分区：
文献类型：
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作者：
Fathima, Aafreen;Rao, Jonnalagadda Raghava;Unni Nair, Balachandran
背景技术背景：从铬污染的土壤（制革污泥）中分离的芽孢杆菌属细菌菌株被用作从制革废水中去除Cr（III）的财团。高岭土是一种天然吸附剂，被用作细菌生物膜形成的支撑材料。粘土支持的细菌生物膜的使用以前没有被用于处理制革废水中含有Cr（III）salt.Results：商业制革废水中含有1000 ppm的初始金属离子浓度进行处理的阶段。在第四阶段之后，将1000 ppm的初始Cr（III）浓度降低至2ppm，这是排放的容许水平。经16s rRNA基因测序鉴定，菌株为枯草芽孢杆菌VITSCCr01和蜡样芽孢杆菌VITSCCr02。批式分析和共聚焦激光扫描显微镜结果显示高岭土作为生物膜形成的支持材料的作用。Freundlich吸附等温线得到最佳拟合。结论：高岭土和细菌等廉价吸附剂对制革废水中Cr（III）的吸附效果较好
BACKGROUND: Bacterial strains belonging to the genus Bacillus, isolated from Cr- polluted soil (tannery sludge) were employed as consortium for Cr(III) removal from tannery effluents. Kaolin clay, a natural adsorbent, was used as supporting material for bacterial biofilm formation. The use of clay-supported bacterial biofilm has not previously been employed for the treatment of tannery effluents containing Cr(III) salt.RESULTS: Commercial tannery effluent containing 1000 ppm initial metal ion concentration was treated in stages. The initial Cr(III) concentration of 1000 ppm was brought down to 2 ppm, a permissible level for discharge, after the fourth stage. The bacterial isolates were found to be Bacillus subtilis VITSCCr01 and Bacillus cereus VITSCCr02 by 16s rRNA gene sequencing. Batch assay and confocal laser scanning microscopy results revealed the role of kaolin as a support material in biofilm formation. Best fit was obtained with the Freundlich adsorption isotherm. Themechanism of sorption was confirmed by Fourier transform infrared (FT-IR) spectroscopy and scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDS).CONCLUSION: Cr(III) removal from tannery effluent using low cost adsorbents such as kaolin and bacteria proved to be effective for metal concentrations