Effect of low temperature between fractionated-dose irradiation doses on mating of the West Indian sweetpotato weevil, Euscepes postfasciatus. (Coleoptera : Curculionidae)
Effect of low temperature between fractionated-dose irradiation doses on mating of the West Indian sweetpotato weevil, Euscepes postfasciatus. (Coleoptera : Curculionidae)
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分次剂量辐照剂量之间的低温对西印度甘薯象甲 Euscepes postfasciatus 交配的影响。
DOI：
10.1007/s13355-011-0087-1
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发表时间：
2012
影响因子：
1.3
通讯作者：
Dai Haraguchi
中科院分区：
文献类型：
--
作者：
Norikuni Kumano;Takashi Kuriwada;Keiko Shiromoto;Dai Haraguchi
昆虫不育技术（SIT）的有效性取决于释放的不育雄性与野生雌性交配和授精的能力，但使用γ辐射诱导不育对不育昆虫的体细胞和生殖细胞都有负面影响。最近，分次剂量辐照（FI），其中灭菌剂量是随着时间的推移，在一系列的小辐照，已被证明在西印度甘薯weevilEuscepes postfasciatus（费尔梅尔）的有效性。FI改善男性交配倾向与急性照射相比，然而，这种更新的技术需要很长的时间（72小时，在目前的情况下）相比，传统的技术（约20分钟），使用单剂量照射（150戈伊），以充分消毒这种象鼻虫。FI所需的额外时间可能会对释放的不育雄性的质量产生负面影响，因为象鼻虫在此期间自由交配会消耗有限的资源，如代谢能或精子。我们评估了与正常条件（25°C）相比，在低温（5°C和15°C）下暂时储存象鼻虫是否改善了不育雄性的质量。在低温下暂时储存在FI提高男性交配倾向。例如，暴露于15°C的雄性的性活跃期至少在辐照后18天。这一时期比正常男性（14天）长。同时，这种方式延缓了雄性生殖发育，暂时降低了交配竞争能力。如果考虑到不育雄性暴露于15°C的长期活跃期，这些缺点将被抵消。我们讨论了在使用SIT forE的根除程序中，低温暂时储存的FI的优势。后筋膜肌
The effectiveness of the sterile insect technique (SIT) depends on the ability of released sterile males to mate with and inseminate wild females, but the use of gamma radiation to induce sterility negatively affects both somatic and reproductive cells of the sterilized insects. Recently, the effectiveness of fractionated-dose irradiation (FI), in which a sterilizing dose is delivered over time in a series of small irradiations, has been demonstrated in the West Indian sweetpotato weevilEuscepes postfasciatus(Fairmaire). FI improves male mating propensity compared with acute irradiation; however, this renewed technique takes a long time (72 h in the present circumstances) compared with the traditional technique (approximately 20 min) using single-dose irradiation (150 Gy) to fully sterilize this weevil. The extra time required by FI might negatively affect the quality of released sterile males, because weevils expend limited resources, such as metabolic energy or sperm, in mating freely in this period. We evaluated whether the temporal storage of weevils at low temperature (5°C and 15°C) improves the quality of sterile males compared with the normal condition (25°C). Temporal storage at low temperature in FI improves male mating propensity. For example, the sexually active phase of males exposed to 15°C was 18 days at least after irradiation. This period was longer than that of normal males (14 days). Meanwhile, this manner delayed male reproductive development and temporarily reduced mating competitiveness ability. If considering the long active phase of sterile males exposed to 15°C, these disadvantage would be cancelled out. We discuss the advantage of FI with temporal storage at low temperature in the eradication program using SIT forE. postfasciatus.