INVIVO COMPLEMENTATION OF INFECTIOUS TRANSCRIPTS FROM MUTANT TOBACCO MOSAIC-VIRUS CDNAS IN TRANSGENIC PLANTS
INVIVO COMPLEMENTATION OF INFECTIOUS TRANSCRIPTS FROM MUTANT TOBACCO MOSAIC-VIRUS CDNAS IN TRANSGENIC PLANTS
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DOI：
10.1016/0042-6822(91)90475-q
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发表时间：
1991-03-01
期刊：
影响因子：
3.7
通讯作者：
BEACHY, RN
中科院分区：
文献类型：
--
作者：
HOLT, CA;BEACHY, RN
构建了烟草花叶病毒（TMV）U1株系的全长cDNA克隆，并利用噬菌体T7 RNA聚合酶在体外产生了高感染性的转录本。移码突变被设计为导致翻译的提前终止被引入到30-kDa的运动蛋白（MP）基因或外壳蛋白（CP）基因。MP移码突变体不能局部或全身感染接种的烟草植株。然而，接种表达野生型TMV MP基因的转基因烟草植物导致局部和全身病毒感染。CP移码突变体，虽然不能移动系统在非转化烟草，表现出系统的运动在转基因植物表达野生型TMV CP基因。因此，表达适当的野生型TMV基因的转基因烟草植物能够互补，反式，缺乏功能性MP或CP基因的突变病毒。
A full-length cDNA clone of the U1 (common) strain of tobacco mosaic virus (TMV) was constructed, and highly infectious transcripts were producedin vitrousing bacteriophage T7 RNA polymerase. Frameshift mutations designed to cause premature termination of translation were introduced into either the 30-kDa movement protein (MP) gene or the coat protein (CP) gene. The MP-frameshift mutant was unable to locally or systemically infect inoculated tobacco plants. However, inoculation of transgenic tobacco plants that expressed a wild-type TMV MP gene resulted in both local and systemic viral infection. The CP-frameshift mutant, although unable to move systemically in nontransformed tobacco, exhibited systemic movement in transgenic plants that expressed a wild-type TMV CP gene. Transgenic tobacco plants that expressed the appropriate wild-type TMV gene were thus able to complement, intrans, mutant viruses lacking a functional MP or CP gene.