Multilayer polyelectrolyte films functionalized by insertion of defensin: A new approach to protection of implants from bacterial colonization
Multilayer polyelectrolyte films functionalized by insertion of defensin: A new approach to protection of implants from bacterial colonization
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DOI：
10.1128/aac.48.10.3662-3669.2004
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发表时间：
2004-10-01
影响因子：
4.9
通讯作者：
Egles, C
中科院分区：
文献类型：
--
作者：
Etienne, O;Picart, C;Egles, C
植入材料的细菌感染是假体手术后最严重的并发症之一。在本研究中，我们开发了一种新的策略的基础上插入的抗菌肽（防御素从冈比亚按蚊蚊子）到多层膜的交替沉积的聚阴离子和聚阳离子。石英晶体微量天平和流动电位测量被用来遵循一步一步的多层膜的构造和膜内的防御素的嵌入。用两种菌株进行抗菌试验：藤黄微球菌（革兰氏阳性菌）和大肠杆菌D22（革兰氏阴性菌）。E.发现当在膜结构中插入10个抗微生物肽层时，在防御素功能化膜的表面处的大肠杆菌D22生长为98%。值得注意的是，只有当带正电荷的聚（L-赖氨酸）是膜的最外层时，才能实现生物功能化。基于通过共聚焦或电子显微镜观察到的细菌粘附实验的结果，这些观察结果可能是由于细菌与膜的带正电荷末端的密切相互作用，这使得防御素与细菌膜结构相互作用。这些结果为在任何类型的可植入生物材料上使用这种容易构建和功能化的结构开辟了新的可能性。改性表面对微生物感染具有活性，并代表了一种新的局部宿主保护手段。
Infection of implanted materials by bacteria constitutes one of the most serious complications following prosthetic surgery. In the present study, we developed a new strategy based on the insertion of an antimicrobial peptide (defensin from Anopheles gambiae mosquitoes) into polyelectrolyte multilayer films built by the alternate deposition of polyanions and polycations. Quartz crystal microbalance and streaming potential measurements were used to follow step by step the construction of the multilayer films and embedding of the defensin within the films. Antimicrobial assays were performed with two strains: Micrococcus luteus (a gram-positive bacterium) and Escherichia coli D22 (a gram-negative bacterium). The inhibition of E. coli D22 growth at the surface of defensin-functionalized films was found to be 98% when 10 antimicrobial peptide layers were inserted in the film architecture. Noticeably, the biofunctionalization could be achieved only when positively charged poly(L-lysine) was the outermost layer of the film. On the basis of the results of bacterial adhesion experiments observed by confocal or electron microscopy, these observations could result from the close interaction of the bacteria with the positively charged ends of the films, which allows defensin to interact with the bacterial membrane structure. These results open new possibilities for the use of such easily built and functionalized architectures onto any type of implantable biomaterial. The modified surfaces are active against microbial infection and represent a novel means of local host protection.