Detector-Free Photothermal Bar-Chart Microfluidic Chips (PT-Chips) for Visual Quantitative Detection of Biomarkers
Detector-Free Photothermal Bar-Chart Microfluidic Chips (PT-Chips) for Visual Quantitative Detection of Biomarkers
复制标题
用于生物标志物视觉定量检测的无探测器光热条形图微流控芯片 (PT-Chips)
DOI：
10.1021/acs.analchem.1c01323
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发表时间：
2021
影响因子：
7.4
通讯作者：
Li, Xiujun
中科院分区：
文献类型：
--
作者：
Zhou, Wan;Fu, Guanglei;Li, Xiujun
由化学反应产生的气体驱动的体积条形图微流控芯片（V-Chips）为即时（POC）视觉生物标志物定量提供了一个有前途的平台。然而，在传统的V型芯片中遇到多种限制，例如昂贵且复杂的芯片制造、复杂的芯片组装以及气体产生的不精确可控性。在此，我们将纳米材料介导的光热效应引入V-Chip，并首次开发了一种新型的V-Chip，光热条形图微流控芯片（PT-Chip），用于可视化定量检测生化物质，而无需任何笨重和昂贵的分析仪器。免疫传感信号通过光热效应（芯片上条形图移动）转换为视觉读出信号，使得能够在具有芯片上标尺的低成本聚合物混合PT芯片上进行定量生物标志物检测。使用PT-芯片同时检测了含有前列腺特异性抗原（PSA）作为模型分析物的4种不同的人血清样品，检测限为2.1 ng/mL，满足临床诊断要求。虽然没有使用传统的信号检测器，它实现了相当的检测灵敏度与吸光度测量与酶标仪。通过测试人全血进一步验证了PT芯片，没有颜色干扰问题，证明了我们的方法即使在复杂基质中也具有良好的分析性能，因此有可能填补目前临床诊断中无法测试全血的差距。这种由纳米材料介导的光热效应驱动的新型PT芯片开辟了微流体平台的新视野，用于在护理点进行无仪器诊断。
The volumetric bar-chart microfluidic chips (V-Chips) driven by chemical reaction-generated gas provide a promising platform for point-of-care (POC) visual biomarker quantitation. However, multiple limitations are encountered in conventional V-Chips, such as costly and complex chip fabrication, complicated chip assembly, and imprecise controllability of gas production. Herein, we introduced nanomaterial-mediated photothermal effects to V-Chips, and for the first time developed a new type of V-Chip, photothermal bar-chart microfluidic chip (PT-Chip), for visual quantitative detection of biochemicals without any bulky and costly analytical instruments. Immunosensing signals were converted to visual readout signals via photothermal effects, the on-chip bar-chart movements, enabling quantitative biomarker detection on a low-cost polymer hybrid PT-Chip with on-chip scale rulers. Four different human serum samples containing a prostate-specific antigen (PSA) as a model analyte were detected simultaneously using the PT-Chip, with a limit of detection of 2.1 ng/mL, meeting clinical diagnostic requirements. Although no conventional signal detectors were used, it achieved comparable detection sensitivity to absorbance measurements with a microplate reader. The PT-Chip was further validated by testing human whole blood without the color interference problem, demonstrating the good analytical performance of our method even in complex matrices and thus the potential to fill the gap in current clinical diagnostics that is incapable of testing whole blood. This new PT-Chip driven by nanomaterial-mediated photothermal effects opens a new horizon of microfluidic platforms for instrument-free diagnostics at the point-of-care.