Intracellularly inducible, ubiquitin hydrolase-insensitive tandem ubiquitins inhibit the 26S proteasome activity and cell division
Intracellularly inducible, ubiquitin hydrolase-insensitive tandem ubiquitins inhibit the 26S proteasome activity and cell division
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DOI：
10.1266/ggs.79.77
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发表时间：
2004-04-01
影响因子：
1.1
通讯作者：
Toh-e, A
中科院分区：
文献类型：
--
作者：
Saeki, Y;Isono, E;Toh-e, A
我们构建了含有泛素串联重复且对泛素水解酶不敏感的多泛素衍生物。它们被指定为串联泛素 (tUb) 以及重复次数，例如 tUb2。当 tUb 在野生型酵母菌株中的 GAL1 启动子控制下表达时，生长受到强烈抑制。在这些条件下，N端规则底物、UFD底物和Gcn4的降解受到抑制，表明tUb抑制26S蛋白酶体活性。与此一致的是，tUb 与 26S 蛋白酶体结合。我们发现 tUb 抑制 26S 蛋白酶体对多泛素化 Sic1 的体外降解。当 tUB6 信使 RNA 注射到非洲爪蟾胚胎中时，细胞分裂受到抑制，这表明 tUb 可用作 26S 蛋白酶体的多功能抑制剂。
We constructed polyubiquitin derivatives that contain a tandem repeat of ubiquitins and were insensitive to ubiquitin hydrolases. They were designated tandem ubiquitin (tUb) with the number of repeats, such as tUb2. When tUbs were expressed under the control of the GAL1 promoter in the wild-type yeast strain, growth was strongly inhibited. Under these conditions, the degradation of N-end rule substrates, a UFD substrate and Gcn4 was inhibited, indicating that the tUb inhibits 26S proteasome activity. Consistent with this, tUb binds to the 26S proteasome. We showed that tUb inhibited the in vitro degradation of polyubiquitinylated Sic1 by the 26S proteasome. When tUB6 messenger RNA was injected into Xenopus embryos, cell division was inhibited, suggesting that tUb can be used as a versatile inhibitor of the 26S proteasome.