Diversity analysis of intestinal microflora between healthy and diarrheal neonatal piglets from the same litter in different regions
Diversity analysis of intestinal microflora between healthy and diarrheal neonatal piglets from the same litter in different regions
复制标题
不同地区同窝健康仔猪和腹泻仔猪肠道菌群多样性分析
DOI：
10.1016/j.anaerobe.2018.12.001
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发表时间：
2019-02-01
期刊：
影响因子：
2.3
通讯作者：
Zhang, Jinhua
中科院分区：
文献类型：
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作者：
Han, Chunsheng;Dai, Yimin;Zhang, Jinhua
虽然新生仔猪腹泻的问题是众所周知的，但健康仔猪和腹泻仔猪在细菌多样性和群落组成方面的差异仍不清楚。我们调查了来自江西的新生仔猪中国的这些差异。从6个地区选取健康仔猪(H，n=20)和腹泻仔猪(D，n=20)。对粪便微生物群落进行16S rRNA基因V3-V4区序列分析。我们发现7个门中主要的门(梭杆菌、拟杆菌、蠕形杆菌和变形杆菌)所占的比例为99%。总体α多样性指数，如CHO、SOBS、盖度和香农等差异不显著。此外，预测功能的相对丰度在两组中高度相似。结果表明，梭状芽孢杆菌可分为两大类群：感觉严格梭菌_1和感觉严格梭菌_2。感觉严格梭菌_2在D组高度丰富，在H组低丰度，而感觉严格意义_1的结果则相反。H组和D组的比较分析表明，属水平上的志贺氏菌和链球菌与种水平上未分类的乳杆菌有显著差异。两组间的比较分析表明，未归类的普氏菌和莫氏梭杆菌在属水平上差异显著，线性判别分析(LDA)得分较高。在排除区域影响的基础上，同一凋落物的微生物组成结果也存在显著差异。这些结果表明仔猪腹泻与这些微生物密切相关。这项研究对肠道微生物的相互作用和仔猪腹泻的预防提供了见解。(C)2018爱思唯尔有限公司。保留所有权利。
Though the problem of neonatal piglet diarrhea is well known, the differences in the bacterial diversity and community composition between healthy and diarrheal piglets are still unknown. We investigated these differences in neonatal piglets from Jiangxi Province, China. Healthy (H, n = 20) and diarrheal (D, n = 20) piglets were selected from six regions. The fecal microbial communities were analyzed by sequencing the V3-V4 region of 16S rRNA gene. We found the ratio of major phyla (Fusobacteria, Bacteroidetes, Firmicutes and Proteobacteria) was >99% of 7 phyla. The overall alpha diversity indices, such as chao, sobs, coverage and Shannon, were not significantly different. Moreover, the relative abundance of the predicted functions was highly similar in the two groups. Our results indicated that Clostridium was divided into two major groups: Clostridium sensu stricto_1 and stricto_2. Sensu stricto_2 was highly abundant in the D group and low abundance in the H group, whereas the results of sensu stricto_1 were opposite. Comparative analyses within the H or D groups showed that Escherichia-Shigella and Streptococcus at the genus level and unclassified Lactobacillus at the species level were significant difference. Comparative analyses of the two groups showed that unclassified Prevotellaceae at the genus level and Fusobacterium mortifierum were significantly different and had high linear discriminant analysis (LDA) scores. The significantly different microbes composition results also existed in the same litter, based on excluding regions influence. These results suggested that piglet diarrhea was closely associated with these microbes. This study provides insights into gut microbial interactions and prevention of piglet diarrhea. (C) 2018 Elsevier Ltd. All rights reserved.