Progesterone Inhibits 20α-Hydroxysteroid Dehydrogenase Expression in the Rat Corpus Luteum through the Glucocorticoid Receptor.
Progesterone Inhibits 20α-Hydroxysteroid Dehydrogenase Expression in the Rat Corpus Luteum through the Glucocorticoid Receptor.
复制标题
黄体酮通过糖皮质激素受体抑制大鼠黄体中 20α-羟基类固醇脱氢酶的表达。
作者：
N. Sugino;C. Telleria;G. Gibori
In this study, we investigated whether progesterone exerts an intraluteal action despite the lack of progesterone receptors (PR) in the rat corpus luteum and whether progesterone acts through the glucocorticoid receptor (GR) to enhance its own levels by down-regulating the expression of 20α-hydroxysteroid dehydrogenase (20α-HSD). We first established that the corpus luteum constitutively expresses the GR throughout pregnancy and after parturition. We also generated a temperature sensitive SV-40 transformed luteal cell line (GG-CL) that expresses the GR and 20α-HSD but lacks the PR. Treatment with different doses of either progesterone or dexamethasone caused a dose-related decrease in 20α-HSD mRNA in both cultured corpora lutea and in the luteal cell line. RU486, a PR/GR antagonist, completely blocked both the progesterone and the dexamethasone mediated inhibition of 20α-HSD expression in GG-CL cells. In summary, this report provides the first evidence that despite the absence of the PR in the rat corpus ...
