Local modulation of plus-end transport targets herpesvirus entry and egress in sensory axons
Local modulation of plus-end transport targets herpesvirus entry and egress in sensory axons
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DOI：
10.1073/pnas.0404686101
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发表时间：
2004-11-09
影响因子：
11.1
通讯作者：
Enquist, LW
中科院分区：
文献类型：
--
作者：
Smith, GA;Pomeranz, L;Enquist, LW
许多病毒的核心结构通过与宿主细胞骨架运动蛋白结合在细胞内移动。然而，在感染的不同阶段，细胞内病毒颗粒被转运至复制位点或细胞外周的机制仍有待了解。通过每秒多帧跟踪轴突内的单个病毒衣壳来检查感觉神经元中疱疹病毒定向运输的调节。进入轴突后，衣壳独立于内体而向细胞体进行双向和跳跃运动。将进入运输与先前对出口期间衣壳轴突运输的分析进行比较表明，衣壳靶向进出细胞是通过调节正端运动而不是负端运动来实现的。进入运输不受先前感染中流出病毒存在的干扰，这表明运输方向不是由病毒基因表达全局调节的，而是直接由病毒颗粒的成分调节的。
The core structures of many viruses move within cells by association with host cytoskeletal motor proteins; however, the mechanisms by which intracellular viral particles are transported toward sites of replication or the cell periphery at distinct stages of infection remain to be understood. The regulation of herpesvirus directional transport in sensory neurons was examined by tracking individual viral capsids within axons at multiple frames per s. After entry into axons, capsids underwent bidirectional and saltatory movement to the cell body independently of endosomes. A comparison of entry transport to a previous analysis of capsid axonal transport during egress revealed that capsid targeting in and out of cells occurs by modulation of plus-end, but not minus-end, motion. Entry transport was unperturbed by the presence of egressing virus from a prior infection, indicating that transport direction is not modulated globally by viral gene expression, but rather directly by a component of the viral particle.