Analysis of eukaryotic translation in purified and semipurified systems.
Analysis of eukaryotic translation in purified and semipurified systems.
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DOI：
10.1002/0471143030.cb1109s08
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发表时间：
2001-05-01
影响因子：
--
通讯作者：
Hensold, J O
中科院分区：
文献类型：
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作者：
Merrick, W C;Hensold, J O
目前对翻译起始所涉及的顺序步骤的大部分了解都是使用蔗糖梯度分离核糖体和核糖体亚基获得的，如本文所述。这些纯化的组分与纯化的翻译因子结合，分析翻译起始中间体的形成和翻译因子在体外的作用。
Much of the current understanding of the sequential steps involved in translation initiation has been obtained using sucrose gradients to isolate ribosomes and ribosomal subunits, as described here. These purified components are combined with purified translation factors to analyze the formation of intermediates in translation initiation and the roles of the translation factors in vitro.