Generation and efficacy evaluation of a recombinant adenovirus expressing the E2 protein of classical swine fever virus
Generation and efficacy evaluation of a recombinant adenovirus expressing the E2 protein of classical swine fever virus
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DOI：
10.1016/j.rvsc.2009.06.005
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发表时间：
2010-02-01
影响因子：
2.4
通讯作者：
Qiu, Hua-Ji
中科院分区：
文献类型：
--
作者：
Sun, Yuan;Liu, Da-Fei;Qiu, Hua-Ji
猪瘟病毒（CSFV）是猪瘟（CSF）的病原体，给全球养猪业造成重大经济损失。CSFV的E2糖蛋白是中和抗体的主要靶标。本研究旨在构建表达猪瘟病毒E2基因的重组人5型腺病毒（rAdV-E2），并评价其在兔和猪体内的免疫效果。结果表明，rAdV-E2免疫家兔和猪后均产生了高水平的CSFV特异性中和抗体。rAdV-E2免疫家兔，对致病性C株感染引起的发热有保护作用; rAdV-E2免疫猪，对强毒石门株的致死攻击有保护作用。这表明重组腺病毒是一种有吸引力的预防CSF的候选疫苗。(C)2009爱思唯尔有限公司版权所有。
Classical swine fever virus (CSFV) is the causative agent of classical swine fever (CSF), which causes significant economic losses to the pig industry worldwide. The E2 glycoprotein of CSFV is the main target for neutralizing antibodies. This study was aimed to develop a recombinant human adenovirus type 5 expressing the CSFV E2 gene (rAdV-E2) and evaluate its efficacy in rabbits and pigs. The results showed that the rabbits and the pigs immunized with the rAdV-E2 developed high-level CSFV-specific neutralizing antibodies. The rAdV-E2-immunized rabbits were protected from fever induced by infection with C-strain, which is pathogenic to the rabbit, and the rAdV-E2-immunized pigs were protected from lethal challenge with highly virulent Shimen strain. This indicates that the recombinant adenovirus can be an attractive candidate vaccine for preventing CSF. (C) 2009 Elsevier Ltd. All rights reserved.