THE EFFECT OF HIGH GLUCOSE AND OXIDATIVE STRESS ON LENS METABOLISM, ALDOSE REDUCTASE, AND SENILE CATARACTOGENESIS
THE EFFECT OF HIGH GLUCOSE AND OXIDATIVE STRESS ON LENS METABOLISM, ALDOSE REDUCTASE, AND SENILE CATARACTOGENESIS
复制标题
DOI：
10.1016/0026-0495(86)90180-0
复制
发表时间：
1986-04-01
影响因子：
9.8
通讯作者：
GONZALEZ, RG
中科院分区：
文献类型：
--
作者：
CHENG, HM;GONZALEZ, RG
糖尿病性白内障的发生是一个多因素的过程，用核磁共振（NMR）检查。P-31 NMR光谱研究表明糖尿病透镜中能量和膜代谢均发生了实质性改变。在35.5 mmol/L葡萄糖中孵育的晶状体中，通过C-13 NMR光谱测定山梨醇途径通量的结果显示：（1）消耗的总葡萄糖的三分之一通过该途径，（2）NADPH向NADP的周转率为3，000%/hr。此外，证实了醛糖还原酶和谷胱甘肽还原酶之间对NADPH的竞争。值得注意的是，高血糖/糖尿病镜片中的所有代谢变化均可通过醛糖还原酶抑制剂（例如，山梨醇）预防。
Diabetic cataractogenesis, a multifactorial process, was examined with nuclear magnetic resonance (NMR). P-31 NMR spectroscopic studies showed substantial alteration of both energy and membrane metabolism in the diabetic lens. Findings from a C-13 NMR spectroscopic determination of the sorbitol pathway flux in lenses incubated in 35.5 mmol/L glucose revealed that (1) one-third of total glucose consumed was channeled through this pathway, and (2) the turnover rate of NADPH to NADP was 3,000%/hr. Furthermore, a competition for NADPH between aldose reductase and glutathione reductase was demonstrated. It is important to note that all metabolic changes in hyperglycemic/diabetic lenses can be prevented by aldose reductase inhibitors, eg, sorbinil.