The bacterial community of entomophilic nematodes and host beetles.
The bacterial community of entomophilic nematodes and host beetles.
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DOI：
10.1111/mec.13614
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发表时间：
2016-05
影响因子：
4.9
通讯作者：
Hong RL
中科院分区：
文献类型：
--
作者：
Koneru SL;Salinas H;Flores GE;Hong RL
昆虫是真核生物中物种最丰富的谱系，每种昆虫都是来自多个门的生物体的潜在宿主，包括真菌、原生动物、螨虫、细菌和线虫。特别是，已知甲虫与不同的细菌群落和昆虫线虫有关。虽然昆虫病原线虫需要共生细菌来杀死昆虫宿主并在其体内繁殖，但促进其他类型线虫-昆虫关联的微生物生态学在很大程度上尚不清楚。为了阐明三营养甲虫-线虫-细菌关系的详细模式，我们调查了大洛杉矶地区五年内圣甲虫的线虫侵染概况，发现某些甲虫宿主的独特线虫侵染模式。在一个季节中，我们使用 16S rRNA 基因的高通量测序来表征甲虫及其相关线虫的细菌群落。我们发现五种常见甲虫宿主物种之间的细菌群落组成存在显着差异，与地理起源无关。厌氧菌增效菌科和硫酸盐还原脱硫弧菌科在Amblonoxia甲虫中最为丰富，而肠杆菌科和毛螺菌科在环头甲虫中常见。与携带细菌共生体的昆虫病原线虫不同，昆虫相关线虫不会改变甲虫的原生细菌群落，其微生物组也不会根据线虫或甲虫宿主物种的不同而有所不同。双腹线虫与 Melolonthinae 甲虫和硫酸盐还原细菌关联的保守性表明甲虫细菌群落与其相关线虫之间可能存在联系。我们的研究结果为了解交通便利的城市环境中土壤大型无脊椎动物与其微生物群之间的动态相互作用奠定了基础。
Insects form the most species-rich lineage of Eukaryotes and each is a potential host for organisms from multiple phyla, including fungi, protozoa, mites, bacteria, and nematodes. In particular, beetles are known to be associated with distinct bacterial communities and entomophilic nematodes. While entomopathogenic nematodes require symbiotic bacteria to kill and reproduce inside their insect hosts, the microbial ecology that facilitates other types of nematode-insect associations is largely unknown. To illuminate detailed patterns of the tritrophic beetle-nematode-bacteria relationship, we surveyed the nematode infestation profiles of scarab beetles in the greater Los Angeles area over a five-year period and found distinct nematode infestation patterns for certain beetle hosts. Over a single season, we characterized the bacterial communities of beetles and their associated nematodes using high-throughput sequencing of the 16S rRNA gene. We found significant differences in bacterial community composition among the five prevalent beetle host species, independent of geographic origin. Anaerobes Synergistaceae and sulfate-reducing Desulfovibrionaceae were most abundant in Amblonoxia beetles, while Enterobacteriaceae and Lachnospiraceae were common in Cyclocephala beetles. Unlike entomopathogenic nematodes that carry bacterial symbionts, insect-associated nematodes do not alter the beetles’ native bacterial communities, nor do their microbiomes differ according to nematode or beetle host species. The conservation of Diplogastrid nematodes associations with Melolonthinae beetles and sulfate-reducing bacteria suggests a possible link between beetle bacterial communities and their associated nematodes. Our results establish a starting point towards understanding the dynamic interactions between soil macroinvertebrates and their microbiota in a highly accessible urban environment.