Detection of MCP-4 in dermal fibroblasts and its activation of the respiratory burst in human eosinophils.
Detection of MCP-4 in dermal fibroblasts and its activation of the respiratory burst in human eosinophils.
复制标题
真皮成纤维细胞中 MCP-4 的检测及其对人嗜酸性粒细胞呼吸爆发的激活。
作者：
H. Petering;R. Höchstetter;D. Kimmig;R. Smolarski;Alexander Kapp;J. Elsner
CC-趋化因子是一个重要的促炎介质家族，可促进慢性炎症疾病中人类嗜酸性粒细胞的募集和激活。最近，据报道，一种新型人类 CC 趋化因子，单核细胞趋化蛋白 4 (MCP-4)，其氨基酸序列与嗜酸细胞趋化因子和 RANTES 相似，诱导嗜酸性粒细胞趋化，并通过特定趋化因子受体发出信号。在这项研究中，我们研究了 MCP-4 对导致呼吸爆发激活的不同嗜酸性粒细胞效应功能的影响。在人嗜酸性粒细胞中，MCP-4 剂量依赖性地诱导活性氧的产生和肌动蛋白聚合作为相关事件。用与信号转导级联相互作用的不同酶抑制剂预处理嗜酸性粒细胞，结果表明 Gi 蛋白、蛋白激酶 C、酪氨酸激酶和磷脂酰肌醇 3 激酶参与 MCP-4 刺激后的信号传导。此外，细胞因子刺激的人真皮成纤维细胞表达高水平的MCP-4 mRNA，表明成纤维细胞是MCP-4的生理来源。因此，本研究表明MCP-4在嗜酸性粒细胞的激活中具有重要作用，并且真皮成纤维细胞和人嗜酸性粒细胞之间的相互作用可能在细胞因子网络中发挥重要作用。
CC-chemokines are an important family of proinflammatory mediators that promote the recruitment and activation of human eosinophils in chronic inflammatory diseases. Recently, a novel human CC-chemokine, monocyte chemotactic protein 4 (MCP-4), has been reported that shows amino acid sequence similarities with eotaxin and RANTES, induces chemotaxis of eosinophils, and signals through specific chemokine receptors. In this study, we investigated the effect of MCP-4 on different eosinophil effector functions leading to the activation of the respiratory burst. In human eosinophils, MCP-4 dose dependently induced the production of reactive oxygen species and actin polymerization as a related event. Pretreatment of eosinophils with different enzyme inhibitors interacting with the signal transduction cascade revealed that Gi protein, protein kinase C, tyrosine kinase, and phosphatidylinositol-3-kinase are involved in the signaling following stimulation with MCP-4. In addition, cytokine-stimulated human dermal fibroblasts expressed high levels of MCP-4 mRNA, suggesting that fibroblasts are a physiologic source of MCP-4. Therefore, this study demonstrates that there is an important role of MCP-4 in the activation of eosinophils and that the interaction between dermal fibroblasts and human eosinophils may play an important role within the cytokine network.
嗜酸性粒细胞粘附至血管细胞粘附分子-1可激活超氧阴离子的产生。
DOI：
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发表时间：
1995
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Nagata,M;Sedgwick,JB;Bates,ME;Kita,H;Busse,WW
通讯作者：
Busse,WW
影响因子：
15.9
作者：
Ponath, PD;Qin, SX;Mackay, CR
通讯作者：
Mackay, CR