Efficient Assessment of Developmental, Surgical and Pathological Lymphangiogenesis Using a Lymphatic Reporter Mouse and Its Embryonic Stem Cells.
Efficient Assessment of Developmental, Surgical and Pathological Lymphangiogenesis Using a Lymphatic Reporter Mouse and Its Embryonic Stem Cells.
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DOI：
10.1371/journal.pone.0157126
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Hong YK
中科院分区：
文献类型：
--
作者：
Hong M;Jung E;Yang S;Jung W;Seong YJ;Park E;Bramos A;Kim KE;Lee S;Daghlian G;Seo JI;Choi I;Choi IS;Koh CJ;Kobielak A;Ying QL;Johnson M;Gardner D;Wong AK;Choi D;Hong YK
最近开发了几个淋巴管报告小鼠系，以显著改善淋巴管的成像。尽管如此，淋巴管直接可视化的使用还没有得到充分的探索和记录。在这里，我们描述了一个新的Prox1-tdTomato转基因淋巴管报告小鼠系，并展示了这种动物工具如何使研究人员能够通过直接显示淋巴管来有效地评估发育、手术和病理淋巴管的生成。此外，我们还从该报告系中获得了胚胎干细胞，并在体内成功地将其分化为淋巴管。总之，这些实验工具和技术将有助于推进淋巴研究。
Several lymphatic reporter mouse lines have recently been developed to significantly improve imaging of lymphatic vessels. Nonetheless, the usage of direct visualization of lymphatic vessels has not been fully explored and documented. Here, we characterized a new Prox1-tdTomato transgenic lymphatic reporter mouse line, and demonstrated how this animal tool enables the researchers to efficiently assess developmental, surgical and pathological lymphangiogenesis by direct visualization of lymphatic vessels. Moreover, we have derived embryonic stem cells from this reporter line, and successfully differentiated them into lymphatic vessels in vivo. In conclusion, these experimental tools and techniques will help advance lymphatic research.