Composition of Microbial Oral Biofilms during Maturation in Young Healthy Adults
Composition of Microbial Oral Biofilms during Maturation in Young Healthy Adults
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DOI：
10.1371/journal.pone.0087449
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发表时间：
2014-02-04
期刊：
影响因子：
3.7
通讯作者：
Schmitz, Ruth A.
中科院分区：
文献类型：
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作者：
Langfeldt, Daniela;Neulinger, Sven C.;Schmitz, Ruth A.
在本研究中，我们旨在分析年轻健康成年人不同成熟阶段口腔生物膜的细菌群落结构。在 5 个不同的成熟间隔（1、3、5、9 和 14 天）后，从 32 名人类受试者身上收集了在膜过滤器上建立的口腔生物膜，并通过 16S rDNA 扩增子测序分析了各自的系统发育多样性。我们的分析揭示了高度多样化的整个定植谱，分布在 8 个门/候选分区和 15 个不同的细菌类别中。观察到受试者微生物群存在巨大的个体间差异，占总方差的 35%，但 α 和 β 多样性缺乏明显的总体时间趋势。我们进一步获得了强有力的证据，表明可以根据三个不同发生且相互排斥的物种簇将受试者分为三个簇。
In the present study we aimed to analyze the bacterial community structure of oral biofilms at different maturation stages in young healthy adults. Oral biofilms established on membrane filters were collected from 32 human subjects after 5 different maturation intervals (1, 3, 5, 9 and 14 days) and the respective phylogenetic diversity was analyzed by 16S rDNA amplicon sequencing. Our analyses revealed highly diverse entire colonization profiles, spread into 8 phyla/candidate divisions and in 15 different bacterial classes. A large inter-individual difference in the subjects' microbiota was observed, comprising 35% of the total variance, but lacking conspicuous general temporal trends in both alpha and beta diversity. We further obtained strong evidence that subjects can be categorized into three clusters based on three differently occurring and mutually exclusive species clusters.