Matrix metalloproteinases have a role in palatogenesis
Matrix metalloproteinases have a role in palatogenesis
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DOI：
10.1177/154405910208101206
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发表时间：
2002-12-01
影响因子：
7.6
通讯作者：
Sandy, JR
中科院分区：
文献类型：
--
作者：
Brown, NL;Yarram, SJ;Sandy, JR
哺乳动物的腭发育取决于腭架抬高、内侧边缘上皮（MEE）分解和间充质流动。这些都需要基质重塑，这部分是由基质金属蛋白酶（MMP）家族控制的。我们使用器官培养系统来检查一般MMP抑制剂（BB 3103）对小鼠腭发育的影响。在20 μ M BB 3103中培养的腭不含活性MMP-2，并且从15个样品大小中只有一个腭融合。在这个单一的腭，MMP-3是目前在更高的水平比腭未能融合。已知MMP-3参与上皮间质转化（EMT），其持续存在可以解释为什么腭融合。这意味着MMPs在正常的腭裂发生中的作用，其活性的破坏可能导致腭裂。
Mammalian palatogenesis depends on palatal shelf elevation, medial edge epithelium (MEE) breakdown, and mesenchyme flow. These all require matrix remodeling, which is controlled in part by the family of matrix metalloproteinases (MMPs). We used an organ culture system to examine the effect of a general MMP inhibitor (BB3103) on mouse palatogenesis. Palates cultured in 20 muM BB3103 contained no active MMP-2, and only one palate fused from a sample size of 15. In this single palate, MMP-3 was present at higher levels than in palates that failed to fuse. MMP-3 is known to be involved in epithelial mesenchymal transformation (EMT), and its persistence may explain why this palate fused. This implies a role for MMPs in normal palatogenesis, and disruption of their activity may result in cleft palate.