The periodontopathic bacteria in placenta, saliva and subgingival plaque of threatened preterm labour and preterm low birth weight cases: A longitudinal study in Japanese pregnant women.
The periodontopathic bacteria in placenta, saliva and subgingival plaque of threatened preterm labour and preterm low birth weight cases: A longitudinal study in Japanese pregnant women.
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先兆早产和早产低出生体重病例的胎盘、唾液和龈下菌斑中的牙周病细菌：一项针对日本孕妇的纵向研究。
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发表时间：
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影响因子：
3.4
通讯作者：
Yuichi Izumi
中科院分区：
文献类型：
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作者：
Changchang Ye;Sayaka Katagiri;Naoyuki Miyasaka;Hiroaki Kobayashi;Thatawee Khemwong;Toshiyuki Nagasawa;Yuichi Izumi
目的：本研究测定了先兆早产（TPL）和早产低出生体重（PLBW）受试者唾液、龈下菌斑和胎盘中牙周致病菌的数量，以确定与不良妊娠结局高度相关的特定牙周病原体。
方法：我们使用TaqMan探针实时荧光定量PCR和酶联免疫吸附测定（ELISA）检测28例TPL患者和36例健康孕妇龈下菌斑、唾液和胎盘组织中伴放线聚集杆菌、牙龈卟啉单胞菌、福赛坦氏菌、齿垢密螺旋体、具核梭杆菌和中间普氏菌的数量，以及针对这些细菌的血清IgG滴度。
结果：64例分娩中有13例产下PLBW婴儿。在胎盘样本中检测到所有6种牙周致病菌。TPL组胎盘样本中具核梭杆菌的数量以及齿垢密螺旋体的检出频率显著高于健康组。同时，TPL - PLBW分娩者的年龄、血清中抗牙龈卟啉单胞菌IgG、菌斑样本中牙龈卟啉单胞菌和福赛坦氏菌的数量、唾液中间普氏菌的检出频率以及牙周袋探诊深度≥5mm的百分比高于TPL - 健康分娩（HD）组和/或健康 - HD组。有序逻辑回归分析显示，胎盘组织中存在具核梭杆菌与TPL显著相关。而母亲年龄与TPL中的PLBW显著相关。
结论：我们的研究结果表明所有6种细菌都可能进入胎盘。胎盘中具核梭杆菌的增加可能与TPL有关。而母亲年龄较大可能与TPL中的PLBW有关。
临床相关性：应在高危人群中应用牙周治疗以减少深牙周袋位点和牙周病原体的定植。
OBJECTIVES: This study determined the quantity of periodontopathic bacteria in saliva, subgingival plaque and placenta on the threatened preterm labor (TPL) and preterm low birth weight (PLBW) subjects in order to identify specific periodontal pathogens with high association to adverse pregnancy outcomes..METHODS: We used real-time PCR with TaqMan probe and ELISA to detect the amount of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Fusobacterium nucleatum, and Prevotella intermedia in subgingival plaque, saliva, and placenta tissue, in addition to serum IgG titers against these bacteria in 28 patients with TPL and 36 healthy pregnant women. .RESULTS: Thirteen of 64 births delivered PLBW infants. All 6 periodontopathic bacteria were detected in the placenta samples. The amount of F. nucleatum and detection frequency of T.denticola in placental samples was significantly higher in the TPL group than in the healthy group. Meanwhile, the age, anti-P. gingival IgG in serum, amount of P. gingivalis and T. forsythia in plaque samples, detection frequency of P. intermedia in saliva and percentage of pocket probing depth ≥ 5 mm were higher in TPL-PLBW births than those in TPL-Healthy delivery (HD) group and/or in H-HD group. Ordinal logistic regression analysis revealed that the presence of F. nucleatum in placental tissues was significantly associated with TPL. While, the maternal age was significantly associated with PLBW in TPL..CONCLUSION: Our findings suggested all 6 bacteria may access the placenta. The increased presence of F. nucleatum in placenta might be related to TPL. While advanced maternal age might be associated with PLBW in TPL..CLINICAL RELEVANCE: Periodontal therapy should be applied to reduce the deep periodontal pocket sites and the colonization of periodontal pathogens in high risk population..