Evaluation of MicroRNA 125b as a potential biomarker for postmenopausal osteoporosis
Evaluation of MicroRNA 125b as a potential biomarker for postmenopausal osteoporosis
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DOI：
10.4314/tjpr.v16i3.20
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发表时间：
2017-03-01
影响因子：
0.6
通讯作者：
Guo, Song
中科院分区：
文献类型：
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作者：
Chen, Hua;Jiang, Honghui;Guo, Song
目的：鉴定中国妇女绝经后骨质疏松症中显著失调的mirna，并检测这些mirna是否具有作为绝经后骨质疏松症循环生物标志物的诊断潜力。方法：招募30例骨质疏松症患者和30例非骨质疏松症健康人，采集血液和骨组织标本。miRNA表达谱分析和定量实时聚合酶链反应（qRT-PCR）用于鉴定和证实骨质疏松症患者血清和骨组织中miRNA的失调。进行受试者工作特征曲线（ROC）分析，以评估显著失调的mirna的诊断潜力。结果：基于谱分析和qRT-PCR， miR-125b、miR-30和miR-5914在绝经后骨质疏松患者的血清和骨组织中显著上调。在所有进行的实验中，与其他上调的mirna相比，在骨质疏松症患者中，miR-125b在血清和骨组织中均表现出最高水平的上调。ROC分析显示，在上调的mirna中，miR-125b的AUC最高。结论：miR-125b是绝经后骨质疏松症中显著上调最高的miRNA。此外，循环miR-125b有可能成为绝经后骨质疏松症的非侵入性生物标志物。
Purpose: To identify significant dysregulated miRNAs in postmenopausal osteoporosis in Chinese women and to test whether any of these miRNAs have diagnostic potential as circulatory biomarkers for postmenopausal osteoporosis.Methods: Thirty osteoporotic patients and 30 non-osteoporotic healthy individuals were recruited, and blood and bone tissue samples were collected from them. miRNA expression profiling and quantitative real-time polymerase chain reaction (qRT-PCR) were used to identify and substantiate dysregulated miRNAs in blood sera and bone tissue from osteoporotic patients. Receiver operating characteristic curve (ROC) analysis was carried out to assess the diagnostic potential of significantly dysregulated miRNAs.Results: Based on profiling and qRT-PCR, miR-125b, miR-30 and miR-5914 were significantly upregulated in the blood sera and bone tissues of patients with postmenopausal osteoporosis. In all the experiments carried out, miR-125b showed the highest levels of upregulation both in the blood sera and bone tissue compared to other upregulated miRNAs in osteoporotic patients. ROC analysis indicate that the AUC of miR-125b was the highest amongst the upregulated miRNAs.Conclusion: miR-125b is the highest significantly upregulated miRNA in postmenopausal osteoporosis. Furthermore, circulating miR-125b has the potential of a non-invasive biomarker for postmenopausal osteoporosis.