Susceptibility of carnivore hosts to strains of canine distemper virus from distinct genetic lineages
Susceptibility of carnivore hosts to strains of canine distemper virus from distinct genetic lineages
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DOI：
10.1016/j.vetmic.2011.10.009
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发表时间：
2012-04-23
影响因子：
3.3
通讯作者：
East, Marion L.
中科院分区：
文献类型：
--
作者：
Nikolin, Veljko M.;Wibbelt, Gudrun;East, Marion L.
利用血凝素（HA）全基因和部分磷蛋白(P)基因对德国梅克伦堡-前波莫瑞州两种野生动物犬瘟热病毒（CDV）进行基因型分析。系统发育分析表明，2007年获得的浣熊（原犬科）毒株与2008年获得的红狐（犬科）毒株存在显著差异。浣熊系属于CDV欧洲野生谱系，而红狐系属于CDV欧洲谱系。我们将我们的基因序列数据与全球138个CDV染色的已发表数据结合起来，研究CDV HA蛋白SLAM结合区530 （G/E到R/D/N）和549 （V到H）的氨基酸替换对家养狗适应的CDV毒株向其他食肉动物传播的重要性。我们没有发现氨基酸530受到宿主物种强烈影响的证据。相反，530位点在CDV谱系中是保守的，与宿主物种无关。与预期相反，来自非狗宿主的菌株没有表现出对预测替代Y549H的偏好。野生犬宿主更常被549Y毒株感染，这种模式与家养狗相似。非犬科菌株在549位点对H和Y没有明显的偏向，但有向549H的倾向。在野生犬类毒株和非犬类毒株中，549Y和549H的流行率存在显著差异，这表明病毒对这些类别的宿主有一定程度的适应。(C) 2011 Elsevier B.V.版权所有
Using the complete haemagglutinin (HA) gene and partial phosphoprotein (P) gene we investigated the genotype of canine distemper virus (CDV) strains recovered from two wildlife species in Mecklenburg-Vorpommern, Germany. Phylogenetic analyses demonstrated significant differences between the strains from raccoons Procyon lotor (family Procyonidae) obtained in 2007 and strains from red foxes Vulpes vulpes (family Canidae) obtained in 2008. The raccoon strains belonged to the CDV European wildlife lineage whereas the red fox strains belonged to the CDV Europe lineage. We combined our genetic sequence data with published data from 138 CDV stains worldwide to investigate the proposed importance of amino acid substitutions in the SLAM binding region of the CDV HA protein at position 530 (G/E to R/D/N) and 549 (V to H) to the spread of domestic dog-adapted CDV strains to other carnivores. We found no evidence that amino acid 530 was strongly affected by host species. Rather, site 530 was conserved within CDV lineages, regardless of host species. Contrary to expectation, strains from non-dog hosts did not exhibit a bias towards the predicted substitution Y549H. Wild canid hosts were more frequently infected by strains with 549Y, a pattern similar to domestic dogs. Non-canid strains showed no significant bias towards either H or Y at site 549, although there was a trend towards 549H. Significant differences between the prevalence of 549Y and 549H in wild canid strains and non-canid strains suggests a degree of virus adaptation to these categories of host. (C) 2011 Elsevier B.V. All rights reserved.