Stress-induced F-Box protein-coding gene OsFBX257 modulates drought stress adaptations and ABA responses in rice.
Stress-induced F-Box protein-coding gene OsFBX257 modulates drought stress adaptations and ABA responses in rice.
复制标题
胁迫诱导的 F-Box 蛋白编码基因 OsFBX257 调节水稻的干旱胁迫适应和 ABA 反应。
DOI：
10.1111/pce.14496
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Sharma E
中科院分区：
文献类型：
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作者：
Sharma E
F-box (FB) 蛋白构成 SKP1-CUL1-F-box (SCF) 型 E3 泛素连接酶的一部分，是植物生长和发育的重要组成部分。在这里，我们表征了 OsFBX257，这是一种水稻 FB 蛋白编码基因，在干旱条件和其他非生物胁迫下差异表达。群体基因组学分析表明OsFBX257在种质中表现出较高的等位基因多样性，并在一些粳稻、芳香和粳稻品种中处于正选择状态。有趣的是，OsFBX257 栽培品种 Nagina22 的等位基因变异与可变剪接转录本相关。 OsFBX257 在陆地植物中保守，是 SCF 复合体的一个组成部分，可以形成同聚物并与 14-3-3 水稻蛋白 GF14b 和 GF14c 发生分子相互作用。OsFBX257 在涉及蛋白激酶和磷酸酶的网络中共表达。我们发现 OsFBX257 可以结合激酶 OsCDPK1 和 OsSAPK2，并且其磷酸化可以被磷酸酶 OsPP2C08 逆转。OsFBX257 表达水平调节水稻的根结构和干旱胁迫耐受性。OsFBX257 敲除 (OsFBX257KD) 品系显示总根长度和深度、冠根数量、穗大小和胁迫下存活率降低。相反，其过表达（OsFBX257OE）会增加水稻的根部深度、叶片和籽粒长度、穗数和籽粒产量。OsFBX257是减轻干旱胁迫引起的水稻损害的有前景的育种靶标。
F‐box (FB) proteins that form part of SKP1‐CUL1‐F‐box (SCF) type of E3 ubiquitin ligases are important components of plant growth and development. Here we characterizedOsFBX257, a rice FB protein‐coding gene that is differentially expressed under drought conditions and other abiotic stresses. Population genomics analysis suggest thatOsFBX257shows high allelic diversity inausaccessions and has been under positive selection in somejaponica,aromaticandindicacultivars. Interestingly, allelic variation atOsFBX257inauscultivar Nagina22 is associated with an alternatively spliced transcript. Conserved among land plants, OsFBX257 is a component of the SCF complex, can form homomers and interact molecularly with the 14‐3‐3 rice proteins GF14b and GF14c.OsFBX257is co‐expressed in a network involving protein kinases and phosphatases. We show that OsFBX257 can bind the kinases OsCDPK1 and OsSAPK2, and that its phosphorylation can be reversed by phosphatase OsPP2C08.OsFBX257expression level modulates root architecture and drought stress tolerance in rice.OsFBX257knockdown (OsFBX257KD) lines show reduced total root length and depth, crown root number, panicle size and survival under stress. In contrast, its overexpression(OsFBX257OE) increases root depth, leaf and grain length, number of panicles, and grain yield in rice.OsFBX257is a promising breeding target for alleviating drought stress‐induced damage in rice.