Clinical significance of Ki67 and circulating tumor cells with an epithelial-mesenchymal transition phenotype in non-small cell lung cancer
Clinical significance of Ki67 and circulating tumor cells with an epithelial-mesenchymal transition phenotype in non-small cell lung cancer
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Ki67和具有上皮间质转化表型的循环肿瘤细胞在非小细胞肺癌中的临床意义
DOI：
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发表时间：
2020
影响因子：
2.2
通讯作者：
Mingwu Chen
中科院分区：
文献类型：
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作者：
Huajian Peng;Xiang Tan;Yongyong Wang;Lei Dai;Guanbiao Liang;Jianji Guo;Mingwu Chen
循环肿瘤细胞（CTC）是具有不同临床和生物学特性的异质肿瘤细胞群。本研究的目的是评估 CTC 与上皮间质转化表型 (CTC EMT) 和增殖标志物 Ki67 之间的关系，及其在非小细胞肺癌 (NSCLC) 中的预后价值。采用CanPatrolTM CTC富集方法从84例NSCLC患者外周血中分离出CTC，并采用免疫组化法检测肿瘤组织中Ki67的表达情况。近三分之二（61/84）的患者CTC EMT呈阳性，55例（65.4%）患者肿瘤组织中Ki67原位高表达（≥14%）。 CTC EMT与肿瘤大小和分化、年龄、性别和组织学类型无显着相关性，但与淋巴转移、肿瘤分期和Ki67过表达相关。此外，与阴性患者相比，CTC EMT+ NSCLC 患者的无复发生存期 (RFS) 和总生存期 (OS) 显着较低。同样，Ki67 水平≥ 14% 与显着较低的 RFS 和 OS 相关。总之，CTC EMT与Ki67表达显着相关，是NSCLC的危险因素。
Circulating tumor cells (CTCs) are a heterogeneous population of tumor cells with distinct clinical and biological properties. The aim of the present study was to evaluate the relationship between CTCs with the epithelial-mesenchymal transition phenotype (CTC EMT) and the proliferative marker Ki67, and their prognostic value in non-small cell lung cancer (NSCLC). CTCs were isolated from the peripheral blood of 84 NSCLC patients using the CanPatrolTM CTC enrichment method, and the expression of Ki67 in tumor tissues were detected by immunohistochemistry. Almost two-thirds (61/84) of the patients were positive for CTC EMT, and 55 (65.4%) patients had high in-situ expression of Ki67 (≥ 14%) in the tumor tissues. CTC EMT was not significantly associated with tumor size and differentiation, age, gender and histological type, but correlated with lymphatic metastasis, tumor stage and Ki67 overexpression. Furthermore, the CTC EMT+ NSCLC patients had a significantly lower recurrence-free survival (RFS) and overall survival (OS) compared to the negative patients. Similarly, Ki67 levels ≥ 14% were associated with a significantly lower RFS and OS. In conclusion, CTC EMT is significantly related to Ki67 expression, and is a risk factor of NSCLC.