Organ culture of benign, aging, and hyperplastic human prostate.
Organ culture of benign, aging, and hyperplastic human prostate.
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良性、衰老和增生性人类前列腺的器官培养。
DOI：
10.1002/jemt.1070300403
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Pretlow,TP
中科院分区：
文献类型：
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作者：
Pretlow,TG;Yang,B;Pretlow,TP
人类前列腺的器官培养始于 20 世纪 70 年代，并以 Lasnitzki 及其合作者二十年前在小鼠身上所做的工作为蓝本。在人类前列腺的器官培养中，在培养的前 3-5 天，上皮细胞迅速增加，基质细胞减少。虽然发生了许多表型特性的调节，但这些培养物提供了一种简单而快速的方法，可以在基质细胞明显耗尽的培养组织中获得大量人类前列腺上皮细胞。有一些证据表明，在雄激素存在的情况下，器官培养物可以保持稍微更好的功能状态。然而，大部分证据都不是定量的。大多数前列腺器官培养都是用来自前列腺内未指定位置的组织完成的。以这种方式进行的培养物的解释不如从前列腺内的特定解剖位置进行的培养物的解释完整。如果要有意义地解释培养物，则必须对邻近培养组织的位置进行仔细的病理表征。 © 1995 Wiley-Liss, Inc.
Organ culture of the human prostate began in the 1970s and was modeled after the work of Lasnitzki and her collaborators in the mouse two decades earlier. In organ culture of human prostates, one sees a rapid increase in epithelial cells and decrease in stromal cells during the first 3–5 days of culture. While modulation of many phenotypic properties occurs, these cultures provide a simple and rapid way to achieve large numbers of human prostatic epithelial cells in cultured tissues that are markedly depleted of stromal cells. There is some evidence that organ cultures are maintained in slightly better functional states in the presence of androgens; however, most of this evidence is less than quantitative. Most organ culture of prostates has been accomplished with tissues from unspecified locations within the prostate; interpretation of cultures carried out in this fashion has been less complete than would have been possible if they had been carried out from specific anatomic locations within the prostate. Careful pathological characterization of locations contiguous to the cultured tissue is mandatory if cultures are to be interpreted meaningfully. © 1995 Wiley‐Liss, Inc.