Sex and age result in differential regulation of the renal thiazide-sensitive NaCl cotransporter and the epithelial sodium channel in angiotensin II-infused mice.
Sex and age result in differential regulation of the renal thiazide-sensitive NaCl cotransporter and the epithelial sodium channel in angiotensin II-infused mice.
复制标题
性别和年龄导致血管紧张素 II 输注小鼠肾噻嗪类敏感 NaCl 协同转运蛋白和上皮钠通道的差异调节。
DOI：
10.1159/000252776
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发表时间：
2009
影响因子：
4.2
通讯作者：
Ecelbarger,CarolynM
中科院分区：
文献类型：
--
作者：
Tiwari,Swasti;Li,Lijun;Riazi,Shahla;Halagappa,VeerendraKMadala;Ecelbarger,CarolynM
背景/目的我们研究了年龄和性别对血管紧张素II(Ang II)对血压(BP)反应的影响，并评价了硫氮化物敏感的氯化钠共转运体(NCC)和上皮钠通道(ENaC)的潜在机制。方法雄性和雌性小鼠(∼3月龄或21月龄)分别给予Ang II或对照组7天。年轻雄性、年轻雌性、老年雄性和老年雌性小鼠的平均收缩压(7日龄)分别为161、143、172和157。支持这一BP图谱的全肾免疫印迹变化包括老年女性和老年男性的NCC条带密度在注射Ang II后分别增加了51%和52%，而年轻男性和年轻女性的NCC条带密度分别增加了40%和0%。与年轻雌性小鼠相比，年轻雄性小鼠的β-和γ-ENaC主要条带的减少程度也更大。结论老年小鼠和雄性小鼠对Ang II的敏感性增加可能与NCC的过度活性有关。
Background/AimsWe determined the effects of age and sex on the blood pressure (BP) response to angiotensin II (Ang II) infusion and evaluated the potential mechanistic role of the thiazide-sensitive NaCl cotransporter (NCC) and the epithelial sodium channel (ENaC).MethodsMale and female mice (∼ 3 or 21 months of age) were infused with Ang II or control for 7 days.ResultsMales had a greater BP response to Ang II, somewhat enhanced by aging. Mean systolic BPs (at 7 days) were (mm Hg): 161, 143, 172, and 157 in young male, young female, old male, and old female mice, respectively. Immunoblotting changes in the whole kidney that supported this BP profile included a 51 and 52% increase in NCC band density in the old females and old males (as compared to sex-respective controls) with Ang II infusion, while the young males and young females showed an increase of 40 and 0%, respectively. Young males also had a greater reduction in major bands of β-and γ-ENaC, than did young female mice. The natriuretic response to hydrochlorothiazide supported an increase in activity of NCC with Ang II in aged mice only.ConclusionsIncreased sensitivity to Ang II in aging and male mice may involve overactivity of NCC.