Risk of oral cancer associated with human papillomavirus infection, betel quid chewing, and cigarette smoking in Taiwan - an integrated molecular and epidemiological study of 58 cases
Risk of oral cancer associated with human papillomavirus infection, betel quid chewing, and cigarette smoking in Taiwan - an integrated molecular and epidemiological study of 58 cases
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DOI：
10.1034/j.1600-0714.2002.00129.x
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发表时间：
2002-07-01
影响因子：
3.3
通讯作者：
Chou, MY
中科院分区：
文献类型：
--
作者：
Chen, PCH;Kuo, C;Chou, MY
背景：口腔鳞状细胞癌（OSCC）与人乳头瘤病毒（HPV）6、11、16和18之间的关系尚不确定。以往的报告在方法和结果上各不相同。我们进行了这项研究，使用原位PCR原位杂交（ISH）检测，这被认为是最敏感的方法检测病毒DNA。我们进行了一项流行病学调查的历史嚼槟榔和吸烟，因为这些习惯是常见的在taiwan.Methods：在原位PCR原位杂交从29例口腔鳞癌患者的肿瘤标本和口腔粘膜标本从29例非口腔鳞癌。结果：单因素分析显示，HPV16、HPV18、嚼槟榔、吸烟是高危因素。HPV6和11则没有。多因素分析显示，HPV 16感染（调整比值比= 11.20）和嚼槟榔（调整比值比= 17.06）仍然是OSCC的独立因素。结论：我们的研究结果表明，HPV 16和嚼槟榔是台湾OSCC的两个主要危险因素，表明它们通过不同的机制在OSCC的发病机制。
Background: The association between oral squamous cell carcinoma (OSCC) and human papillomavirus (HPV) 6, 11, 16 and 18 is uncertain. Past reports varied in the methodology and results. We conducted this study using in situ PCR in situ hybridization (ISH) assay which was considered as the most sensitive method for detection of viral DNA. We undertook an epidemiologic survey about the history of betel quid chewing and cigarette smoking, since these habits are common in Taiwan.Methods: In situ PCR ISH was performed on the tumor specimens from 29 patients with OSCC and the oral mucosal specimens from 29 patients without OSCC. Their betel quid chewing and cigarette smoking histories were also reviewed.Results: HPV16, HPV18, betel quid chewing and cigarette smoking were statistically significant risk factors in univariate analysis. HPV6 and 11 were not. Multivariate analysis showed that HPV16 infection (adjusted Odds ratio = 11.20) and betel quid chewing (adjusted Odds ratio = 17.06) remained to be independent factors for OSCC.Conclusions: Our results showed that HPV16 and betel quid chewing were two major risk factors for OSCC in Taiwan, indicating that they act through different mechanisms in the pathogenesis of OSCC.