Conidia immobilization of T-DNA inserted Trichoderma atroviride mutant AMT-28 with dichlorvos degradation ability and exploration of biodegradation mechanism.
Conidia immobilization of T-DNA inserted Trichoderma atroviride mutant AMT-28 with dichlorvos degradation ability and exploration of biodegradation mechanism.
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DOI：
10.1016/j.biortech.2010.07.059
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发表时间：
2010-12
影响因子：
11.4
通讯作者：
Wenliang Sun;Yunpeng Chen;Lixing Liu;Jun Tang;Jie Chen;Peng Liu
中科院分区：
文献类型：
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作者：
Wenliang Sun;Yunpeng Chen;Lixing Liu;Jun Tang;Jie Chen;Peng Liu
采用固定化分生孢子法研究了深绿木霉T-DNA插入突变株AMT-28对敌敌畏的降解能力。每100 mL海藻酸钠溶液中固定化孢子数为107个时，降解能力最强。固定化分生孢子的降解能力比固定化或自由悬浮的菌丝体有明显的提高。固定化细胞具有良好的贮存能力和重复使用性。经HPLC分析，AMT-28菌丝体在7天内可完全去除敌敌畏。敌敌畏在营养缺陷型Burk培养基中的降解速率受氮源的影响较大。AMT-28对敌敌畏的去除主要是通过生物矿化作用实现的。
An immobilizing conidia approach was used to study the degradation ability of dichlorvos in Trichoderma atroviride T-DNA insertional mutant AMT-28. Beads with 107immobilized conidia per 100mL of Na-alginate solution exhibited the highest degradation abilities. The immobilized conidia showed enhanced degradation abilities compared with immobilized or freely suspended mycelia. The immobilized cells kept good storage capacity and reusability. Dichlorvos was confirmed to be completely removed by mycelia of AMT-28 within 7 days using HPLC analysis. The dichlorvos degradation rates in auxotrophic Burk media varied and were significantly affected by nitrogen sources. There was no detectable biosorption and the removal of dichlorvos in AMT-28 was primarily attributed to a kind of Biomineralization process.