ISOLATION OF NUCLEAR-PORE COMPLEXES IN ASSOCIATION WITH A LAMINA
ISOLATION OF NUCLEAR-PORE COMPLEXES IN ASSOCIATION WITH A LAMINA
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DOI：
10.1073/pnas.72.3.1007
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发表时间：
1975-01-01
影响因子：
11.1
通讯作者：
BLOBEL, G
中科院分区：
文献类型：
--
作者：
AARONSON, RP;BLOBEL, G
通过去污剂和盐分级法从大鼠肝脏核膜中分离出与150 A厚层相关的核孔复合物。孔复合物表现出特征性的形态，并出现在一个高度特定的方向连接到薄层，它在相对较大的面积上延伸。孔复合物-层组分由三种主要多肽和几种次要多肽组成，具有很少或没有DNA、RNA或磷脂。这表明，孔复合物和椎板的协会反映了在体内的核周边的结构安排。
Nuclear pore complexes have been isolated in association with a 150 A thick lamina by detergent and salt fractionation of nuclear envelopes from rat liver. The pore complexes exhibit characteristic morphology and appear to be attached in a highly specific orientation to the lamina, which extends over relatively large areas. The pore complex-lamina fraction is composed of three major and several minor polypeptides with little or no DNA, RNA, or phospholipid. It is suggested that the association of the pore complexes and the lamina reflects the structural arrangement of the nuclear periphery in vivo.