Identification of glucagon-producing cells (A cells) in dog gastric mucosa.
Identification of glucagon-producing cells (A cells) in dog gastric mucosa.
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DOI：
10.1083/jcb.69.2.455
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发表时间：
1976-05
期刊：
影响因子：
--
通讯作者：
Orci L
中科院分区：
文献类型：
--
作者：
Baetens D;Rufener C;Srikant BC;Dobbs R;Unger R;Orci L
使用特异性抗胰高血糖素血清的免疫细胞化学技术显示，胰高血糖素细胞仅位于狗胃的氧合腺粘膜中。粘膜电镜检查显示内分泌细胞含有分泌颗粒，圆形致密的核周围有一个清晰的晕，与胰腺a细胞的分泌颗粒难以区分。与胰腺A细胞的α颗粒一样，经Grimelius银染色后，胃内分泌细胞颗粒呈银颗粒外周分布。此外，利用过氧化物酶免疫细胞化学技术在超微结构水平上发现这些细胞颗粒被反应产物特异性标记。因此，这些细胞被命名为胃A细胞。这些数据表明，胃氧合粘膜包含与胰腺A细胞在细胞学、细胞化学和免疫细胞化学上难以区分的细胞。据信胃A细胞负责胃胰高血糖素的分泌。
An immunocytochemical technique using specific antiglucagon serum reveals the presence of glucagon-containing cells situated exclusively in the oxyntic glandular mucosa of the dog stomach. Electron microscope examination of the mucosa demonstrated endocrine cells containing secretory granules with a round dense core surrounded by a clear halo, indistinguishable from secretory granules of pancreatic A cells. Like the alpha granules of pancreatic A cells, the granules of these gastric endocrine cells exhibited a peripheral distribution of silver grains after Grimelius silver staining. Moreover, the granules of these cells were found to be specifically labeled with reaction product, using the peroxidase immunocytochemical technique at the ultrastructural level. Accordingly, these cells were named gastric A cells. These data suggest that the gastric oxyntic mucosa contains cells indistinguishable cytologically, cytochemically, and immunocytochemically from pancreatic A cells. It is believed that gastric A cells are responsible for the secretion of the gastric glucagon.