GLUCOSAMINE RESISTANCE IN YEAST .2. CYTOPLASMIC DETERMINANTS CONFERRING RESISTANCE
GLUCOSAMINE RESISTANCE IN YEAST .2. CYTOPLASMIC DETERMINANTS CONFERRING RESISTANCE
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DOI：
10.1007/bf00264732
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发表时间：
1977-01-01
期刊：
影响因子：
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通讯作者：
BALL, AJS
中科院分区：
文献类型：
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作者：
KUNZ, BA;BALL, AJS
酿酒酵母的两种胞质葡萄糖胺抗性 (GR) 突变体 GR6 和 GR10 与带有已知线粒体 [mit] 标记的菌株杂交。对这些杂交中营养和减数分裂分离模式的分析表明，葡萄糖胺抗性赋予因子并不存在于 mitDNA 上。溴化乙锭治疗证实了这一点，该治疗完全消除了由于 OLI2 基因座线粒体突变引起的寡霉素耐药性，但未能消除同一菌株中存在的 GR 因子。 GR6 和 G410 与其他一些已知的细胞质决定簇的比较表明，虽然葡萄糖胺抗性与杀伤质粒无关，但它可能与 URE3 决定簇和/或 PSI 因子等位。
Two cytoplasmic, glucosamine resistant (GR) mutants of Saccharomyces cerevisiae, GR6 and GR10, were crossed to strains bearing known mitochondrial [mit] markers. Analysis of vegetative and meiotic segregation patterns in these crosses suggested that the glucosamine resistance conferring factor did not reside on mitDNA. This was confirmed by ethidium bromide treatments which completely abolished oligomycin resistance due to a mitochondrial mutation at the OLI2 locus, but which failed to eliminate the GR factor present in the same strain. Comparison of GR6 and G410 to some other known cytoplasmic determinants suggested that while glucosamine resistance was not related to the killer plasmid it may be allelic to the URE3 determinant and/or to the PSI factor.