Growth inhibition of Microcystis aeruginosa by sand-filter prevalent manganese-oxidizing bacterium
Growth inhibition of Microcystis aeruginosa by sand-filter prevalent manganese-oxidizing bacterium
复制标题
砂滤流行锰氧化菌对铜绿微囊藻生长的抑制作用
DOI：
10.1016/j.seppur.2020.117808
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发表时间：
2021-02-01
影响因子：
8.6
通讯作者：
Qu, Jiuhui
中科院分区：
文献类型：
--
作者：
Qi, Jing;Song, Yongjun;Qu, Jiuhui
利用溶藻细菌抑制藻类生长已成为一种有吸引力的方法。然而，以往的研究主要集中在通过添加溶藻细菌来控制富营养化沃茨中的有害水华。本研究旨在探讨藻细胞的生长在砂滤普遍锰氧化细菌的存在下，这可能会减轻藻引起的堵塞问题，砂滤通过生物强化砂滤。锰氧化细菌假单胞菌QJX-1对藻类生长具有抑制作用。与对照组相比，M. 10%QJX-1培养上清可显著降低铜绿假单胞菌的生长，降低幅度达60%。QJX-1无细胞培养上清液对藻细胞光合作用的影响。结果表明，QJX-1对M.通过分泌杀藻化合物2，4-二叔丁基苯酚（2，4-DTBP）而不直接接触藻类细胞来对铜绿假单胞菌进行杀藻。QJX-1可通过2，4-DTBP的分泌破坏藻细胞的光合系统结构，这可以解释QJX-1对藻细胞光合活性的影响。我们的研究结果强调了锰氧化细菌假单胞菌QJX-1对M.铜绿假单胞菌通过2，4-DTBP的排泄。
The utilization of algicidal bacteria is becoming an attractive method for the inhibition of algae growth. However, the previous research has mainly focused on the control of harmful blooms in eutrophic waters by the addition of algicidal bacteria. This study aims to investigate the growth of algal cells in the presence of a sand filter prevalent manganese-oxidizing bacterium, which might alleviate the algae-induced clogging problem in sand filters via bioaugmented sand filtration. The manganese-oxidizing bacterium Pseudomonas sp. QJX-1 exhibited an inhibiting effect on algae growth. Compared with a control group, the chlorophyll a content of M. aeruginosa could be significantly decreased by 60% after exposure to 10% QJX-1 culture supernatant. The decrease in photosynthetic parameters demonstrated the damage to the photosynthetic system of algal cells exposed to QJX-1 cell-free supernatant. The results indicated that QJX-1 inhibited the growth of M. aeruginosa by excreting an algicidal compound, 2,4-di-tert-butylphenol (2,4-DTBP), without directly contacting algal cells. The photosynthetic system structure of algal cells could be damaged through 2,4-DTBP excretion by QJX-1, which could explain the weakened photosynthetic activity of algal cells exposed to QJX-1. Our findings highlight the algicidal effect of the manganese-oxidizing bacterium Pseudomonas sp. QJX-1 on M. aeruginosa via the excretion of 2,4-DTBP.