Prevalence of Helicobacter hepaticus, Murine Norovirus, and Pneumocystis carinii and Eradication Efficacy of Cross-Fostering in Genetically Engineered Mice
Prevalence of Helicobacter hepaticus, Murine Norovirus, and Pneumocystis carinii and Eradication Efficacy of Cross-Fostering in Genetically Engineered Mice
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DOI：
10.1538/expanim.58.497
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发表时间：
2009-10-01
影响因子：
2.4
通讯作者：
Lee, Wang-Jae
中科院分区：
文献类型：
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作者：
Yeom, Su-Cheong;Yu, Sun-A;Lee, Wang-Jae
我们调查了肝螺杆菌，小鼠诺如病毒（MNV），卡氏肺孢子虫的患病率和交叉培养的49个基因工程小鼠（GEM）菌株在我们的研究所根除的疗效。H的流行。肝吸虫、MNV和卡氏肺吸虫感染率分别为33.9%、36.5%和8.6%，免疫缺陷株的感染率高于免疫活性株。此外，相同的免疫表型菌株显示出相似的患病率。NKT细胞在H.肝耐药有趣的是，H. MNV多重感染。单次或多次感染H.选择肝吸虫、MNV和/或卡氏肺吸虫，并进行交叉培养。交叉培育对卡氏肺吸虫的根除是成功的，但对H.与以前的研究相比，根除效果相对较低。我们认为这种低疗效可能是由于持续感染和对H.免疫缺陷GEM株的肝细胞和MNV。因此，交叉培养可能适用于卡氏肺孢子虫的根除，但不适用于H.肝细胞或MNV感染的GEM株。我们的研究结果提供了维护，应变敏感性和成功的再衍生，特别是创业板的基本数据。
We investigated the prevalence of Helicobacter hepaticus, murine norovirus (MNV), and Pneumocystis carinii and the efficacy of cross-fostering for their eradication in 49 genetically engineered mouse (GEM) strains at our institute. Prevalences of H. hepaticus, MNV, and P. carinii were 33.9, 36.5, and 8.6%, respectively, and immunodeficient strains showed relatively higher prevalence of the 3 pathogens than immunocompetent strains. Additionally, the same immune phenotype strains showed similar prevalences. Furthermore, it was found that NKT cells might play a role in H. hepaticus resistance. Interestingly, there was a high incidence of H. hepaticus and MNV multiple infection. Strains with single or multiple infections of H. hepaticus, MNV, and/or P carinii were selected, and cross-fostering was conducted. Cross-fosterings were successful at eradicating P carinii, but there were some failures for H. hepaticus and MNV, and the efficacy of eradication was relatively low compared with previous studies. We thought that this low efficacy might have been due to persistent infection and the high suscepibility to H. hepaticus and MNV of immunodeficient GEM strains. Therefore, cross-fostering may be appropriate for P. carinii eradication, but be inappropriate for repopulation of a new breeding colony with H. hepaticus or MNV infected GEM strains. Our findings provide basic data on maintenance, strain susceptibility, and successful rederivation, especially for GEMs.