Finding better protein engineering strategies
Finding better protein engineering strategies
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DOI：
10.1038/nchembio0809-526
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发表时间：
2009-08-01
影响因子：
14.8
通讯作者：
Bornscheuer, Uwe T.
中科院分区：
文献类型：
--
作者：
Kazlauskas, Romas J.;Bornscheuer, Uwe T.
今天的蛋白质改良策略涉及合理设计与随机诱变和筛选的广泛变化的组合。为了取得更大的进展，即使后续的蛋白质工程问题更容易解决，蛋白质工程师必须严格地比较这些策略，并消除不太有效的策略。
Protein improvement strategies today involve widely varying combinations of rational design with random mutagenesis and screening. To make further progress-defined as making subsequent protein engineering problems easier to solve-protein engineers must critically compare these strategies and eliminate less effective ones.