Regulation of adiponectin receptor R1 and R2 gene expression in adipocytes of C57BL/6 mice
Regulation of adiponectin receptor R1 and R2 gene expression in adipocytes of C57BL/6 mice
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DOI：
10.1016/j.bbrc.2005.02.081
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发表时间：
2005-04-15
影响因子：
3.1
通讯作者：
Paschke, R
中科院分区：
文献类型：
--
作者：
Blüher, M;Fasshauer, M;Paschke, R
脂联素作为胰岛素敏感性的介导剂已经获得了显著的关注。最近，这种脂肪细胞分泌激素的两种受体--脂联素受体I（AdipoR 1）和2（AdipoR 2）已被克隆。为了提高我们对脂肪细胞中这些受体的调节的理解，通过定量实时逆转录聚合酶链反应测量C57 BL/6小鼠的棕色脂肪细胞和来自上性腺和皮下脂肪组织的脂肪细胞中的AdipoR 1和AdipoR 2 mRNA作为进食和生物钟的函数。与随机喂养的小鼠相比，禁食小鼠的棕色脂肪组织和上腹部脂肪组织中的AdipoR 1基因表达较高，但皮下脂肪中的AdipoR 1基因表达较低。与此同时，与进食小鼠相比，禁食小鼠的上腹部脂肪组织中的AdipoR 2 mRNA水平也较高，然而，棕色脂肪和皮下脂肪中的AdipoR 2 mRNA不受进食状态的调节。此外，与皮下脂肪相比，AdipoR 2基因在上性腺中的表达显著更高。有趣的是，一个平行的昼夜节律的基因表达模式与较低的表达在20：00和06：00之间的棕色，上腹部，和皮下脂肪组织AdipoR 1和AdipoR 2也founded. In结论，我们的研究结果表明，脂肪仓库的具体调控的AdipoR 1和AdipoR 2基因表达的棕色和白色脂肪禁食。此外，我们已经确定了一个协调的昼夜节律模式的AdipoR 1和AdipoR 2基因表达在这些组织。(c)2005年爱思唯尔公司All rights reserved.
Adiponectin has gained significant attention as a mediator of insulin sensitivity. Recently, two receptors of this adipocyte-secreted hormone, adiponectin receptor I (AdipoR1) and 2 (AdipoR2), have been cloned. To improve Our understanding of the regulation of these receptors in adipocytes, AdipoR1 and AdipoR2 mRNA was measured by quantitative real-time reverse transcription-polymerase chain reaction in brown adipocytes and adipocytes from epigonadal and subcutaneous adipose tissue of C57BL/6 mice as a function of feeding and circadian clocks. AdipoR1 gene expression was higher in brown adipose tissue and epigonadal adipose tissue, but lower in subcutaneous fat in fasted as compared to random-fed mice. In parallel, AdipoR2 mRNA levels were also higher in epigonadal adipose tissue of fasted as compared to fed mice, however, there was no regulation of AdipoR2 mRNA in brown and subcutaneous fat depending on the feeding state. Furthermore, AdipoR2 gene expression was significantly higher in epigonadal as compared to subcutaneous fat. Interestingly, a parallel circadian gene expression pattern for both AdipoR1 and AdipoR2 with lower expression between 20:00 and 06:00 h in brown, epigonadal, and subcutaneous adipose tissue was also found. In conclusion, our results suggest a fat depot specific regulation of AdipoR1 and AdipoR2 gene expression in brown and white fat by fasting. In addition, we have identified a coordinated circadian pattern of AdipoR1 and AdipoR2 gene expression in these tissues. (c) 2005 Elsevier Inc. All rights reserved.