miR-8 microRNAs regulate the response to osmotic stress in zebrafish embryos.
miR-8 microRNAs regulate the response to osmotic stress in zebrafish embryos.
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DOI：
10.1083/jcb.200807026
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发表时间：
2009-04-06
期刊：
影响因子：
--
通讯作者：
Patton JG
中科院分区：
文献类型：
--
作者：
Flynt AS;Thatcher EJ;Burkewitz K;Li N;Liu Y;Patton JG
微小RNA（miRNAs）是高度保守的小RNA，它们作为基因表达的翻译调节因子，通过选择性地靶向具有互补序列元件的信使RNA（mRNAs）发挥其作用。这些RNA在动物发育和生理的多个方面发挥功能。由于能够在翻译水平上作为快速应答因子，miRNAs也可能影响应激反应。在这项研究中，我们表明miR - 8家族的miRNAs调节斑马鱼胚胎的渗透调节。离子细胞是一种分散在整个表皮的特殊细胞类型，在鳃形成之前的早期发育过程中负责pH值和离子稳态。高度保守的miR - 8家族在离子细胞中表达，并通过调节Nherf1的表达来精确控制离子转运，Nherf1是跨膜离子转运蛋白顶端运输的调节因子。最终，miR - 8家族成员功能的破坏导致无法对渗透应激作出反应，并阻碍在离子细胞顶端表面正确运输和/或聚集跨膜糖蛋白的能力。
MicroRNAs (miRNAs) are highly conserved small RNAs that act as translational regulators of gene expression, exerting their influence by selectively targeting mRNAs bearing complementary sequence elements. These RNAs function in diverse aspects of animal development and physiology. Because of an ability to act as rapid responders at the level of translation, miRNAs may also influence stress response. In this study, we show that the miR-8 family of miRNAs regulates osmoregulation in zebrafish embryos. Ionocytes, which are a specialized cell type scattered throughout the epidermis, are responsible for pH and ion homeostasis during early development before gill formation. The highly conserved miR-8 family is expressed in ionocytes and enables precise control of ion transport by modulating the expression of Nherf1, which is a regulator of apical trafficking of transmembrane ion transporters. Ultimately, disruption of miR-8 family member function leads to an inability to respond to osmotic stress and blocks the ability to properly traffic and/or cluster transmembrane glycoproteins at the apical surface of ionocytes.
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DOI：
10.1007/s10162-002-3022-x
发表时间：
2003-06-01
影响因子：
2.4
作者：
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影响因子：
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DOI：
10.1152/ajpregu.00578.2006
发表时间：
2007-05-01
影响因子：
2.8
作者：
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影响因子：
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作者：
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