Requirement of matrix metalloproteinase-9 for the transformation of human mammary epithelial cells by Stat3-C
Requirement of matrix metalloproteinase-9 for the transformation of human mammary epithelial cells by Stat3-C
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DOI：
10.1073/pnas.0404100101
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发表时间：
2004-07-20
影响因子：
11.1
通讯作者：
Bromberg, JF
中科院分区：
文献类型：
--
作者：
Dechow, TN;Pedranzini, L;Bromberg, JF
在许多不同的癌症中发现了持续激活的Stat 3，包括大约60%的乳腺肿瘤。在这里，我们表明，组成性激活Stat 3转化永生化的人乳腺上皮细胞，这种致癌事件需要基质金属蛋白酶-9（MMP-9）的活性。通过免疫组化分析，我们观察到MMP-9的强表达和酪氨酸磷酸化的Stat 3在原发性乳腺癌标本中呈正相关。这些结果表明活化的Stat 3和MMP-9在乳腺癌发生中的关系。
Persistently activated Stat3 is found in many different cancers, including approximate to60% of breast tumors. Here, we demonstrate that a constitutively activated Stat3 transforms immortalized human mammary epithelial cells and that this oncogenic event requires the activity of matrix metalloproteinase-9 (MMP-9). By immunohistochemical analysis, we observe a positive correlation between strong MMP-9 expression and tyrosine phosphorylated Stat3 in primary breast cancer specimens. These results demonstrate a relationship between activated Stat3 and MMP-9 in breast oncogenesis.