A versatile rapid-mixing and flow device for X-ray absorption spectroscopy.
A versatile rapid-mixing and flow device for X-ray absorption spectroscopy.
复制标题
用于 X 射线吸收光谱的多功能快速混合和流动装置。
DOI：
10.1107/s0909049503028474
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Auld,DavidS
中科院分区：
文献类型：
--
作者：
Zhang,Ke;Liu,Ruifeng;Irving,Tom;Auld,DavidS
设计并实现了一个低温快速混合和流动系统，用于在阿贡国家实验室先进光子源的ID-18光束线上通过X射线吸收光谱监测涉及金属离子的催化。该系统将允许通过X射线荧光检测在稀金属离子浓度下检查生物金属中间体。仪器可以冷却到零度以下，从而延长反应中间体的使用寿命。便携式紫外可见光谱仪与流动系统集成，以光学方式监测样品。该系统还可以用作连续流装置，通过减少样品暴露于X射线束来最大限度地减少辐射引起的样品损坏。同步加速器光束线和X射线荧光检测器系统的集成的停流仪器，使其独特的时间分辨的X射线吸收研究稀释的生物反应。该系统的初步测试的结果。
A low-temperature rapid-mixing and flow system has been designed and implemented to monitor catalysis involving metal ions by X-ray absorption spectroscopy at the ID-18 beamline of the Advanced Photon Source, Argonne National Laboratory. The system will allow examination of biological metallo-intermediates at dilute metal ion concentrations by the detection of X-ray fluorescence. The instrument can be cooled to sub-zero temperatures, thus lengthening the life time of a reaction intermediate. A portable UV–visible spectrometer is integrated with the flow system to monitor the sample optically. The system can also be used as a continuous-flow device to minimize radiation-induced sample damage by reducing sample exposure to the X-ray beam. The integration of the stop-flow instrument with the synchrotron beamline and X-ray fluorescence detector systems makes it unique for time-resolved X-ray absorption studies of dilute biological reactions. The results of the initial testing of the system are presented.