Extraction of phenolic compounds from elder berry and different grape marc varieties using organic solvents and/or supercritical carbon dioxide
Extraction of phenolic compounds from elder berry and different grape marc varieties using organic solvents and/or supercritical carbon dioxide
复制标题
DOI：
10.1016/j.jfoodeng.2008.06.028
复制
发表时间：
2009-01-01
影响因子：
5.5
通讯作者：
Knez, Zeljko
中科院分区：
文献类型：
--
作者：
Vatai, Tuende;Skerget, Mojca;Knez, Zeljko
研究了从葡萄渣和接骨木果中提取酚类物质的最佳工艺条件。目的是获得具有高花青素含量的提取物，其在储存期间显示稳定性，并且作为天然着色剂具有潜在的商业应用价值。为此目的，三个品种的葡萄渣，原产于斯洛文尼亚（雷福斯克，梅洛和解百纳），进行了研究。结果与提取接骨木浆果，这是众所周知的，其高花青素含量。用乙醇、乙酸乙酯和丙酮以不同比例与水进行单步分批提取。施加20 ° C、40 ° C和60 ° C的温度。研究了提取介质pH值变化对花色苷得率和降解率的影响。采用超临界流体萃取（SFE）和常规间歇萃取相结合的两步萃取法。在单步萃取中，最有效的常规溶剂是60 ℃下的有机溶剂和水的混合物。用超临界CO2（有或没有乙醇作为共溶剂）预处理的天然材料，提高了从葡萄渣中提取多酚。该方法提供了植物材料预处理的替代方案，取代了有毒的有机溶剂（例如己烷）。酸化的提取液中花色苷含量较高。但在贮藏过程中花色苷的降解率较高，导致色泽的损失。向提取溶剂中加入市售的迷迭香提取物改善了葡萄渣提取物在储存期间的稳定性。(c)2008爱思唯尔有限公司保留所有权利。
Optimal operating conditions for the extraction of phenolic compounds from grape marc and elder berry have been investigated. The aim was to obtain extracts with high anthocyanin content, which show stability during storage and would be potentially interesting for commercial applications as natural colorants. For this purpose three varieties of grape marc, native to Slovenia (Refosk, Merlot and Cabernet), were studied. The results were compared with the extraction of elder berry, which is known for its high anthocyanin content. Single-step batch extractions were performed with ethanol, ethyl-acetate and acetone in different ratios with water. Temperatures of 20 degrees C, 40 degrees C and 60 degrees C were applied. Influence of the change in pH values of the extraction media was investigated regarding the yield and degradation of anthocyanins. Two-step extractions, combining supercritical fluid extractions (SFE) and conventional batch extractions, were applied. The most efficient conventional solvents in single-step extractions were mixtures of organic solvent and water at 60 degrees C. Pre-treatment of the natural material with supercritical CO2 (with or without ethanol as co-solvent) improved the extraction of polyphenols from the grape marc. This method provides an alternative to the pre-treatment of the plant materials, replacing toxic organic solvents (e.g. hexane). Acidified extraction media resulted in higher anthocyanin content in the extracts. However, the degradation of the anthocyanins during storage was higher, which led to the loss of the intensive colour. The addition of a commercially available rosemary extract to the extraction solvent improved the stability of the grape marc extract during storage. (c) 2008 Elsevier Ltd. All rights reserved.