QUANTITATIVE AND QUALITATIVE ASPECTS ON THE DISTRIBUTION OF 5-HT AND ITS COEXISTENCE WITH SUBSTANCE-P AND TRH IN CAT VENTRAL MEDULLARY NEURONS
QUANTITATIVE AND QUALITATIVE ASPECTS ON THE DISTRIBUTION OF 5-HT AND ITS COEXISTENCE WITH SUBSTANCE-P AND TRH IN CAT VENTRAL MEDULLARY NEURONS
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DOI：
10.1016/0891-0618(94)90003-5
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发表时间：
1994-07-01
影响因子：
2.8
通讯作者：
HOKFELT, T
中科院分区：
文献类型：
--
作者：
ARVIDSSON, U;CULLHEIM, S;HOKFELT, T
用间接免疫荧光技术研究了猫经秋水仙碱处理后，中缝中线核和尾侧脑干外侧网状核（P18.3-P8.5）内5-羟色胺（5-HT）、P物质和促甲状腺素释放激素（TRH）免疫反应（IR）神经元的分布。此外，还用双标记技术分析了5-HT、P物质和TRH样免疫反应（LI）在这些神经元中的共存情况。结果表明，中缝中线核和外侧网状核的胞体含有5-HT、P物质和TRH-LI。5-HT-IR细胞比肽能神经元更丰富，在分析的所有领域。定量估计表明，研究区域内5-HT-IR细胞的总数约为17 × 103，而P物质和TRH-IR细胞的相应数量分别为11 × 103和12 × 103。从双标记切片得出结论，绝大多数肽能细胞也含有5-HT-LI（87-100%）。然而，5-HT-IR神经元的亚群缺乏肽LI（10-55%）。5-HT与肽共存的程度随脑干水平而变化，在更多吻侧位置的神经元显示出较低的5-HT与肽共存的发生率。也可以证明所有三种化合物存在于同一个细胞体中。总之，5-HT-，物质P-和TRH-IR细胞体中遇到的髓核已知含有神经元与脊髓的投影。在这些核中，化合物之间的共存程度很高。所获得的结果与早期研究的5-HT纤维在脊髓中的分布和肽共定位的模式吻合得很好。生化不同的神经元亚群内的5-HT球脊髓通路的存在进行了讨论。
By use of the indirect immunofluorescence technique the distributions of 5-hydroxytryptamine (5-HT)-, substance P- and thyrotropin-releasing hormone (TRH)-immunoreactive (IR) neurons have been studied in the midline raphe nuclei and nucleus reticularis lateralis of the caudal brainstem (levels P18.3-P8.5; according to Berman (1968), in the cat, after treatment with colchicine. In addition, by use of the double-labelling technique, the coexistence between 5-HT-, substance P- and TRH-like immunoreactivity (LI) in these neurons was analysed. The results show that cell bodies in the midline raphe nuclei and nucleus reticularis lateralis contain 5-HT-, substance P- and TRH-LI. 5-HT-IR cells were more abundant than peptidergic neurons in all areas analysed. Quantitative estimations indicated that the total number of 5-HT-IR cells in the regions studied was about 17 x 10(3), while the corresponding numbers for substance P- and TRH-IR cells were 11 x 10(3) and 12 x 10(3), respectively. From double-labelled sections it was concluded that the vast majority of peptidergic cells also contained 5-HT-LI (87-100%). However, a subpopulation of 5-HT-IR neurons lacked peptide-LI (10-55%). The degree of coexistence varied with the brainstem level, in that neurons at more rostral locations showed a lower incidence of coexistence between 5-HT and peptide(s). The presence of all three compounds in one and the same cell body could also be demonstrated. In summary, 5-HT-, substance P- and TRH-IR cell bodies were encountered in medullary nuclei known to contain neurons with projection to the spinal cord. A high degree of coexistence between the compounds was demonstrated in these nuclei. The obtained results fit well with earlier studies on the patterns of distribution and peptide colocalization of 5-HT fibres in the spinal cord. The existence of biochemically distinct neuronal subpopulations within the 5-HT bulbospinal pathway is discussed.