Novel human reovirus isolated from children and its long-term circulation with reassortments
Novel human reovirus isolated from children and its long-term circulation with reassortments
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DOI：
10.1038/s41598-020-58003-9
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发表时间：
2020-01-22
影响因子：
4.6
通讯作者：
Iritani, Nobuhiro
中科院分区：
文献类型：
--
作者：
Yamamoto, Seiji P.;Motooka, Daisuke;Iritani, Nobuhiro
哺乳动物正呼肠孤病毒（MRV），也称为呼肠孤病毒，于20世纪50年代被发现，并成为第一个报道的分段双链RNA病毒。此后，mrv在包括人类在内的多种动物物种中被发现。然而，关于MRV感染的报道很少，因为它们很少出现症状。在日本的监测研究中，mrv自1981年以来一直被检测为胃肠道病原体，共有135例记录。在日本大阪市，MRV于1994年首次从一名脑膜炎儿童身上分离出来，然后在2005年和2014年从肠胃炎儿童身上分离出来。在这里，我们对来自日本的人类MRV分离株进行了首次分子表征，并鉴定出一种属于MRV 2型的新型人类呼肠孤病毒株，命名为MRV-2大阪菌株。该菌株与台湾污水中的MRV-2分离株密切相关，与中国一只蝙蝠的MRV-2分离株相对接近。我们的数据表明，MRV-2大阪毒株在日本人类中传播了至少20年，已在国际上传播。
Mammalian orthoreovirus (MRV), also known as reovirus, was discovered in the 1950s and became the first reported segmented double-stranded RNA virus. MRVs have since been found in a variety of animal species, including humans. However, reports on MRV infections are scarce due to the rarity of their symptomatic occurrence. In Japanese surveillance studies, MRVs have been detected as gastrointestinal pathogens since 1981, with a total of 135 records. In Osaka City, Japan, MRV was first isolated in 1994 from a child with meningitis, and then in 2005 and 2014 from children with gastroenteritis. Here, we conducted the first molecular characterization of human MRV isolates from Japan and identified a novel human reovirus strain belonging to MRV type 2, designated the MRV-2 Osaka strain. This strain, with all three isolates classified, is closely related to MRV-2 isolates from sewage in Taiwan and is relatively close to an MRV-2 isolate from a bat in China. Our data suggest that the MRV-2 Osaka strain, which has circulated amongst humans in Japan for at least two decades, has spread internationally.