Characterisation of an acapsular mutant of Burkholderia pseudomallei identified by signature tagged mutagenesis
Characterisation of an acapsular mutant of Burkholderia pseudomallei identified by signature tagged mutagenesis
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DOI：
10.1099/0022-1317-51-7-539
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发表时间：
2002-07-01
影响因子：
3
通讯作者：
Titball, RW
中科院分区：
文献类型：
--
作者：
Atkins, T;Prior, R;Titball, RW
通过标记诱变方法鉴定了在类鼻疽小鼠模型中减毒的类鼻疽伯克霍尔德氏菌突变体。转座子被证明插入到荚膜生物合成操纵子内的基因中。与野生型细菌相比，该突变体在小鼠模型中的半数致死量增加了10（5）倍，并且它不与针对高摩尔浓度的单克隆抗体反应。wt %的B的多糖。假鼻疽为了确定感染的动力学，将小鼠腹膜内（i.p.）和静脉内（i.v.）突变体和野生型细菌。腹膜内攻击后，腹腔内突变细菌的数量下降，而野生型细菌增殖。当通过静脉途径给药时，突变体能够引起疾病，但与野生型相比，死亡时间增加。对小鼠给予突变体，随后用野生型B激发。pseudomallei，但突变体未能诱导保护性免疫应答。
A Burkholderia pseudomallei mutant which was attenuated in a mouse model of melioidosis was identified by a signature tagged mutagenesis approach. The transposon was shown to be inserted into a gene within the capsular biosynthetic operon. Compared with the wild-type bacteria this mutant demonstrated a 10(5)-fold increase in the median lethal dose in a mouse model and it did not react with a monoclonal antibody against high mol. wt polysaccharide of B. pseudomallei. To determine the kinetics of infection, mice were dosed intraperitoneally (i.p.) and intravenously (i.v.) with, mutant and wildtype bacteria. After i.p challenge, the number of mutant bacteria in the peritoneal cavity declined, whereas wild-type bacteria proliferated. When administered by the i.v. route, the mutant was able to cause disease but the time to death was increased compared with the wild type. Mice were dosed with the mutant and subsequently challenged with wildtype B. pseudomallei, but the mutant failed to induce a protective immune response.