Detection of lymphocytes expressing human T-lymphotropic virus type III in lymph nodes and peripheral blood from infected individuals by in situ hybridization.
Detection of lymphocytes expressing human T-lymphotropic virus type III in lymph nodes and peripheral blood from infected individuals by in situ hybridization.
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通过原位杂交检测感染个体的淋巴结和外周血中表达人类 T 淋巴细胞病毒 III 型的淋巴细胞。
DOI：
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发表时间：
1986
影响因子：
11.1
通讯作者：
F. Wong
中科院分区：
文献类型：
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作者：
M. Harper;L. Marselle;R. Gallo;F. Wong
本文应用原位杂交技术，直接检测了艾滋病（AIDS）患者的原发淋巴结和外周血及AIDS相关复合体中人类T淋巴细胞病毒III型（HTLV-III）的RNA。单核细胞制备物与35 S标记的HTLV-III特异性RNA探针杂交，并暴露于放射自显影乳剂2天。在研究的约86%（6/7）的淋巴结和50%（7/14）的外周血样品中检测到表达病毒RNA的HTLV-III感染细胞。然而，在检查的所有患者样本中，观察到标记细胞的频率非常低（小于总单核细胞的0.01%）。HTLV-III感染的细胞表现出与淋巴细胞一致的形态学特征，并以相对低的丰度（每个细胞20-300个拷贝）表达病毒RNA。这些结果表明，HTLV-III在淋巴结和外周血中的表达在体内非常低。此外，在HTLV-III相关淋巴结病中观察到的淋巴结增生不是直接由于HTLV-III感染的淋巴细胞的增殖。
By using in situ hybridization methodology, we have directly examined primary lymph node and peripheral blood from patients with acquired immunodeficiency syndrome (AIDS) and AIDS-related complex for the presence of human T-lymphotropic virus type III (HTLV-III) viral RNA. Mononuclear cell preparations were hybridized with a 35S-labeled HTLV-III-specific RNA probe and exposed to autoradiographic emulsion for 2 days. HTLV-III-infected cells expressing viral RNA were detected in approximately 86% (6/7) of lymph node and 50% (7/14) of peripheral blood samples studied. However, in all patient samples examined, labeled cells were observed at very low frequency (less than 0.01% of total mononuclear cells). The HTLV-III-infected cells exhibited morphological characteristics consistent with that of lymphocytes and expressed viral RNA at relatively low abundance (20-300 copies per cell). These results demonstrate that HTLV-III expression in lymph node and peripheral blood is very low in vivo. Furthermore, the lymph node hyperplasia observed in HTLV-III-associated lymphadenopathy is not directly due to proliferation of HTLV-III-infected lymphocytes.