Influence of Cellulose Charge on Bacteria Adhesion and Viability to PVAm/CNF/PVAm-Modified Cellulose Model Surfaces
Influence of Cellulose Charge on Bacteria Adhesion and Viability to PVAm/CNF/PVAm-Modified Cellulose Model Surfaces
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DOI：
10.1021/acs.biomac.9b00297
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发表时间：
2019-05-01
影响因子：
6.2
通讯作者：
Wagberg, Lars
中科院分区：
文献类型：
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作者：
Chen, Chao;Petterson, Torbjorn;Wagberg, Lars
基于阳离子表面活性剂在纤维素材料表面上的物理吸附的接触活性抗菌方法如今被认为是产生抗菌表面和材料的环境友好方式。在这种方法中，经处理的表面的静电电荷被认为是细菌吸附和细菌失活/杀灭水平的重要因素。为了阐明纤维素的表面电荷密度对细菌吸附的影响以及对它们的生存能力，细菌被吸附到纤维素模型表面上，其被物理吸附的阳离子聚电解质改性以产生具有不同正电荷密度的表面。通过阳离子聚乙烯胺（PVAm）/阴离子纤维素纳米纤丝/PVAm到初始不同带电的纤维素模型表面上的逐层（LbL）组装来改变表面电荷。暴露后的LBL处理的表面大肠杆菌在水介质中，细菌的吸附以及非活/活细菌的比例和LBL改性纤维素的表面电荷之间存在正相关性。通过仔细的胶体探针原子力显微镜测量，据估计，由于表面电荷的差异，处理过的表面和细菌之间的相互作用力至少为50 nN，可以实现最高表面电荷的表面，并建议这些相当大的相互作用力足以破坏细菌细胞壁，从而杀死细菌。
A contact-active antibacterial approach based on the physical adsorption of a cationic polyelectrolyte onto the surface of a cellulose material is today regarded as an environment-friendly way of creating antibacterial surfaces and materials. In this approach, the electrostatic charge of the treated surfaces is considered to be an important factor for the level of bacteria adsorption and deactivation/killing of the bacteria. In order to clarify the influence of surface charge density of the cellulose on bacteria adsorption as well as on their viability, bacteria were adsorbed onto cellulose model surfaces, which were modified by physically adsorbed cationic polyelectrolytes to create surfaces with different positive charge densities. The surface charge was altered by the layer-by-layer (LbL) assembly of cationic polyvinylamine (PVAm)/anionic cellulose nanofibril/PVAm onto the initially differently charged cellulose model surfaces. After exposing the LbL-treated surfaces to Escherichia coli in aqueous media, a positive correlation was found between the adsorption of bacteria as well as the ratio of nonviable/viable bacteria and the surface charge of the LbL-modified cellulose. By careful colloidal probe atomic force microscopy measurements, it was estimated, due to the difference in surface charges, that interaction forces at least 50 nN between the treated surfaces and a bacterium could be achieved for the surfaces with the highest surface charge, and it is suggested that these considerable interaction forces are sufficient to disrupt the bacterial cell wall and hence kill the bacteria.