Spectral variation of fluorescence lifetime near single metal nanoparticles.
Spectral variation of fluorescence lifetime near single metal nanoparticles.
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DOI：
10.1038/srep21349
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发表时间：
2016-02-15
影响因子：
4.6
通讯作者：
Richards D
中科院分区：
文献类型：
--
作者：
Li J;Krasavin AV;Webster L;Segovia P;Zayats AV;Richards D
我们探索了荧光增强的光谱依赖性以及与单个金属纳米颗粒耦合的荧光分子的相关寿命修正。将荧光寿命成像显微镜和单粒子暗场光谱相结合，研究了单个金纳米粒子的荧光发射光谱与局域表面等离子体(LSP)光谱重叠对荧光寿命缩短的依赖关系。当荧光和LSP共振重合时，观察到最大的寿命缩短，这与数值模拟提供的很好的一致。我们得到的实验和模拟之间的明确比较，提供了对LSP介导的荧光的光谱工程的洞察，并可能导致在传感和生物医学中的优化应用。
We explore the spectral dependence of fluorescence enhancement and the associated lifetime modification of fluorescent molecules coupled to single metal nanoparticles. Fluorescence lifetime imaging microscopy and single-particle dark-field spectroscopy are combined to correlate the dependence of fluorescence lifetime reduction on the spectral overlap between the fluorescence emission and the localised surface plasmon (LSP) spectra of individual gold nanoparticles. A maximum lifetime reduction is observed when the fluorescence and LSP resonances coincide, with good agreement provided by numerical simulations. The explicit comparison between experiment and simulation, that we obtain, offers an insight into the spectral engineering of LSP mediated fluorescence and may lead to optimized application in sensing and biomedicine.