Lignocellulosic materials as solid support agents for Bjerkandera adusta SM46 to enhance polycyclic aromatic hydrocarbon degradation on sea sand and sea water media
Lignocellulosic materials as solid support agents for Bjerkandera adusta SM46 to enhance polycyclic aromatic hydrocarbon degradation on sea sand and sea water media
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DOI：
10.1016/j.bcab.2016.10.011
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发表时间：
2016-10-01
影响因子：
4
通讯作者：
Tachibana, Sanro
中科院分区：
文献类型：
--
作者：
Andriani, Ade;Tachibana, Sanro
利用白色腐真菌（WRF）作为降解剂在极端环境中仍然受到其特定生长要求的限制。研究了一株新分离自日本Saragamine山的WRF菌株Bjerkandera adusta SM 46（GenBank登录号：KU 055648）对多环芳烃（PAHs）污染的海砂和海水的生物修复能力。几种多环芳烃（2-5环）被用作盐碱胁迫条件下的污染物。在四种木质纤维素材料中，即，木粉、木棉纤维、稻草和纸浆废弃物中，稻草是最适合的木质纤维素材料，基于接种B后的真菌生长、木质素降解酶的产生和多环芳烃的降解速率，选择稻草作为最适合的载体。adusta SM46.稻草固定化B。adusta（RSIB）具有更快的生长和定殖，并提高漆酶（Lac）、锰过氧化物酶（MnP）和木质素过氧化物酶（LiP）的活性。稻草作为B固定剂的最佳粒径。adusta为840 μ m。Lac、MnP和LiP活性监测15和30 d。RSIB对低分子量多环芳烃（LMW-PAHs，2-3环）的降解作用最大。当生长在高分子量的多环芳烃（HMW-PAHS，4-5环），降解率变化之间的16%和63%之间的污染海砂和22%和61%之间的污染海水。RSIB也可以用作比浸没培养方法更有效和高效地产生木质素分解酶的替代方法。
The utilization of white rot fungi (WRF) as degrader agents in extreme environments is still limited by their specific growth requirements. This study examined the ability of Bjerkandera adusta SM46 (GenBank accession number: KU055648), a recently isolated WRF from Saragamine mountain, Japan, to treat sea sand and sea water contaminated with polycyclic aromatic hydrocarbons (PAHs) for potential applications in the bioremediation processes. Several PAHs (2-5 rings) were used as pollutants under saline-alkaline stress conditions. Among four lignocellulosic materials, i.e., wood meal, kapok fibre, rice straw, and pulp waste, rice straw was a lignocellulosic material selected as the most suitable support based on the fungal growth, ligninolytic enzymes production, and degradation rates of PAHs after inoculation with B. adusta SM46. Rice straw-immobilized B. adusta (RSIB) showed faster growth and colonization, and increased laccase (Lac), manganese peroxidase (MnP), and lignin peroxidase (LiP) activity. The optimum granule size of rice straw as an immobilizing agent for B. adusta was 840 mu m. Lac, MnP, and LiP activities were monitored for 15 and 30 d. Low-molecular-weight PAHs (LMW-PAHs, 2-3 rings) were the most extensively degraded by RSIB. When grown on high-molecular-weight PAHs (HMW-PAHS, 4-5 rings), degradation rates varied between 16% and 63% on contaminated sea sand and between 22% and 61% on contaminated sea water. RSIB may also be used as an alternative method to more effectively and efficiently produce ligninolytic enzymes than the submerged culture method.