Effects of temperature and relative humidity in D2O on solid-state hydrogen deuterium exchange mass spectrometry (ssHDX-MS)
Effects of temperature and relative humidity in D2O on solid-state hydrogen deuterium exchange mass spectrometry (ssHDX-MS)
复制标题
D2O 中的温度和相对湿度对固态氢氘交换质谱 (ssHDX-MS) 的影响
DOI：
10.1016/j.ijpharm.2021.120263
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发表时间：
2021
影响因子：
5.8
通讯作者：
Topp, Elizabeth M.
中科院分区：
文献类型：
--
作者：
Tukra, Rishabh;Gardner, Sam;Topp, Elizabeth M.
将含有肌红蛋白和各种赋形剂的冻干粉末在不同温度和D2 O蒸气活度（RH）下进行ssHDX-MS。将氘掺入拟合为每种制剂的双指数缔合模型，并评价回归参数对温度和RH的依赖性。数据拟合最好的简化模型中，慢指数项被认为是不变的温度和RH，而快指数项保留其温度和RH的依赖性。这表明，快速的过程，如水蒸气吸附和初始氘标记可能更依赖于温度和RH比缓慢的过程，如顺序交换和运输的固体基质。
Lyophilized powders containing myoglobin and various excipients were subjected to ssHDX-MS at different temperatures and D2O vapor activity (RH). Deuterium incorporation was fitted to a bi-exponential association model for each formulation and the dependence of regression parameters on temperature and RH was evaluated. Data fitted best to a simplified model in which the slow exponential term was considered invariant with temperature and RH while the fast exponential term retained its temperature and RH dependence. This suggests that rapid rate processes such as water vapor sorption and initial deuterium labeling may be more dependent on temperature and RH than slower processes such as sequential exchange and transport in the solid matrix.